Red blood cell & Plts preservation

Question 1

1. What is the maximum volume of blood that can be
   collected from a 110-lb donor, including samples for
   processing?
   a. 450 mL
   b. 500 mL
   c. 525 mL
   d. 550 mL

Answer 1

c. 525 mL

Question 2

2. How often can a blood donor donate whole blood?
   a. Every 24 hours
   b. Once a month
   c. Every 8 weeks
   d. Twice a year

Answer 2

c. Every 8 weeks

Question 3

3. When RBCs are stored, there is a “shift to the left.” This means:

a. Hemoglobin-oxygen affinity increases, owing to an increase in 2,3-DPG.
b. Hemoglobin-oxygen affinity increases, owing to a decrease in 2,3-DPG.
c. Hemoglobin-oxygen affinity decreases, owing to a decrease in 2,3-DPG.
d. Hemoglobin-oxygen affinity decreases, owing to an increase in 2,3-DPG.

Answer 3

b. Hemoglobin-oxygen affinity increases, owing to a decrease in 2,3-DPG.

Question 4

4. The majority of platelets transfused in the United States today are:
   a. Whole blood–derived platelets prepared by the platelet-rich plasma method.
   b. Whole blood–derived platelets prepared by the buffy coat method.
   c. Apheresis platelets.
   d. Prestorage-pooled platelets.

Answer 4

c. Apheresis platelets.

Question 5

5. Which of the following anticoagulant preservatives
   provides a storage time of 35 days at 1°C to 6°C for units
   of whole blood and prepared RBCs if an additive solution
   is not added?
   a. ACD-A
   b. CP2D
   c. CPD
   d. CPDA-1

Answer 5

d. CPDA-1

Question 6

6. What are the current storage time and storage temperature for platelet concentrates and apheresis platelet
   components?
   a. 5 days at 1°C to 6°C
   b. 5 days at 24°C to 27°C
   c. 5 days at 20°C to 24°C
   d. 7 days at 22°C to 24°C

Answer 6

c. 5 days at 20°C to 24°C

Question 7

7. RBCs can be frozen for:
   a. 12 months.
   b. 1 year.
   c. 5 years.
   d. 10 years

Answer 7

d. 10 years

Question 8

8. Whole blood and RBC units are stored at what
   temperature?
   a. 1°C to 6°C
   b. 20°C to 24°C
   c. 37°C
   d. 24°C to 27°C

Answer 8

a. 1°C to 6°C

Question 9

9. Additive solutions are approved for storage of red blood
   cells for how many days?
   a. 21
   b. 42
   c. 35
   d. 7

Answer 9

b. 42

Question 10

10. One criterion used by the FDA for approval of new
    preservation solutions and storage containers is an average 24-hour post-transfusion RBC survival of more than:
    a. 50%.
    b. 60%.
    c. 65%.
    d. 75%

Answer 10

d. 75%

Question 11

11. What is the lowest allowable pH for a platelet component at outdate?
    a. 6
    b. 5.9
    c. 6.8
    d. 6.2

Answer 11

d. 6.2

Question 12

12. Which of the following occurs during storage of red
    blood cells?
    a. pH decreases
    b. 2,3-DPG increases
    c. ATP increases
    d. plasma K+ decreases

Answer 12

a. pH decreases

Question 13

13. Which of the following is approved for bacterial detection specific to extending the expiration of apheresed
    platelets to 7 days?
    a. BacT/ALERT
    b. eBDS
    c. Gram stain
    d. Pan Genera Detection (PGD) test

Answer 13

d. Pan Genera Detection (PGD) test

Question 14

14. Which of the following is the most common cause of
    bacterial contamination of platelet products?
    a. Entry of skin plugs into the collection bag
    b. Environmental contamination during processing
    c. T in the donor
    d. Incorrect storage temperature

Answer 14

a. Entry of skin plugs into the collection bag

Question 15

15. The INTERCEPT pathogen reduction system uses which
    of the following methods?
    a. Riboflavin and UV light
    b. Amotosalen and UV light
    c. Solvent/detergent treatment
    d. Irradiation

Answer 15

b. Amotosalen and UV light