SPIROCHETES Flashcards
Microscopes to visualize spirochetes
Dark field
Fluorescence
Phase Contrast
Loosely Coiled
Borrelia
Tightly coiled w/ corkscrew motility
Treponema
Slightly coiled w/ HOOKED ENDS
Leptospira
Spirochetes:
Arthropod vectors including lice and ticks
“stretched spirals”
Borellia
BORELLIA
Agent: Louse Borne Relapsing Fever
Vector: Human louse (Pediculus humanus)
B. recurrentis (EPIDEMIC)
BORELLIA
Agent: Tick-borne Relapsing Fever
Vector: Ornithodoros ticks
B. anserine, toricatae, parkeri (ENDEMIC)
BORELLIA
Agent: Lyme disease
B. burgdorferi
BORELLIA
B. burgdogferi is transmitted via
Tick vectors IXODES DAMMINI/DEER TICKS
Stages of LYME DISEASE
- Lesion ; Erythrema chronicum
- dissemination through blood
- neurological abnormalities; arthritis
TREPONEMA
Lab Diagnosis
Dark Field - Corkscrew motility
Levaditi silver
Serology
TREPONEMA
Serology:
Screening
Confirmatory
Screening
- VDRL
- RPR
- TRUST (Reagin Test)
Confirmatory
- FTA-ABS
- TPHA
- MHA-TP
HATTS
TREPONEMA
T. pallidum t. pallidum
Venereal Syphilis (Great pox, Evil pox, French/Italian/Spanish)
TREPONEMA
Primary Syphilis
Hard Chancre (painless and firm)
Spx: swab, aspirate
TREPONEMA
Secondary Syphilis
Condylomata lata (wart-like lesions)
Spx: serum
TREPONEMA
Latent Syphilis
Asymptomatic (+) serologic
TREPONEMA
Tertiary syphilis
Neurosyphilis (Tabes dorsalis)
Gummas
Spx: CSF
Screening: VDRL
Confirmatory: FTA-ABS
T. pallidum sub pertenue
Yaws/Framboise “pertenYAWS”
MOT: skin contact with inf. lesion
T. pallidum sub endemicum
Bejel
MOT: mouth to mouth
T. carateum
Pinta “Si cara ay gusto mag pinta”
MOT: skin contact with lesion
TREPONEMA
Destructive lesions of gums
Vincent’s disease
Tightly coiled with hooked ends
Leptospira
LEPTOSPIRA
Nonpathogenic, found in water and soil
L. biflexa
LEPTOSPIRA
Human and animal leptospirosis
Involves: Liver, Kidney, CNS
1 hook
L. interrogans
LEPTOSPIRA
Severe form of Leptospirosis
Weil’s disease
LEPTOSPIRA
Specimen 1st week
Blood/CSF Tissue (Acute)
LEPTOSPIRA
Specimen 2nd week
Urine (Chronic)
LEPTOSPIRA
Culture Media
- Ellinghausen-Mccoulough
- Fletcher’s
- Stuarts
LEPTOSPIRA
Screening
Macroscopic Agglutination Test
serum + Ag (killed lepto) = + agglu
LEPTOSPIRA
Confirmatory
Microscopic Agglutination Test
serum + Ag (live lepto) = + agglu in DF