IV: Gram-negative Bacilli (NF)

Question 1

Non-Fermenting Gram-Negative Bacilli: O-F TEST Composition:

CHO:
pH indicator:
Positive:
Negative:

Answer 1

CHO: Glucose
pH indicator: Bromthymol blue
Positive: Yellow
Negative: Green

Question 2

Common pH indicator:

Answer 2

1. Bromcresol Purple (Purple-yellow)
2. *Andrade’s Acid Fuchsin (Yellow-pink)
3. Phenol Red (Red-yellow)
4. Bromthymol blue (green-yellow)

Question 3

Colorless in Macconkey
With Pigment and Odor
Most are Oxidase Positive
Most are Multi-drug resistant

Answer 3

Gram-negative Bacilli (Non-Fermenters)

Question 4

OF TEST - Fermenters

Open tube:
Closed Tube:

Answer 4

Open tube: yellow
Closed Tube: yellow

Question 5

OF TEST - Oxidizers

Open tube:
Closed Tube:

Answer 5

Open tube: Yellow
Closed Tube: Green

Question 6

Obligate aerobe
Sweet odor; Grape-like odor; Corn-taco like odor
Motile; Rod Shaped

Answer 6

P. aeruginosa

Question 7

Corn-taco agar is due to

Answer 7

2-aminoacetophenone

Question 8

Gram-negative Bacilli (NF)

CPON

Answer 8

S. maltophilia
Acinetobacter

Question 9

Gram-negative Bacilli (NF)

CPOP

Answer 9

Plesiomonas
Burkholderia

Question 10

SPP. ASSOCIATED WITH NOSOCOMIAL INFECTIONS = MDR

Answer 10

1# P. aeruginosa
2# Acinetobacter baumanii
3# S. maltophilia

Question 11

Grows in 37-42 deg Cel
Serrated Confluent Growth
Metallic Green sheen in SBA
Burns = Blue-green pus

Answer 11

P. aeruginosa

Question 11

Associated with Swimmer’s ear infection
Keratitis (contaminated LENS SOLUTION)
Ecthy Gangrenosum/ Hot tub dermatitis

Answer 11

P. aeruginosa

Question 12

causes glander’s disease of horses; nonmotile

Answer 12

Burkholderia mallei

Question 13

Burkholderia mallei with pulmonary involvements is associated in

Answer 13

Horses

Question 14

Burkholderia mallei that is fatal and associated with ulcer of skin and mucous membrane (LYMPANGITIS AND SEPSIS)

Answer 14

Human

Question 15

Also known as Whitmore’s Bacillus

Answer 15

Burkholderia pseudomallei

Question 16

Causes Melioidosis (Vietnamese Time bomb) Glander’s disease

Dry pink, wrinkled colonies in Ashdown colonies

Bipolar inclusion bodies

Earthy Odor

Answer 16

Burkholderia pseudomallei

Question 17

Burkholderia found in soils

Oxidase and LDC (+)

Pseudomonas Cepacia Agar = Pink colonies

Earthy and dirt like odor

Answer 17

B. cepacia

Question 18

B. cepacia is associated with

a. swimmer’s ear
b. vietnamese time bomb
c. Heart valve endocarditis, Foot rot in humans, Onion bulb rot

Answer 18

C

Heart valve endocarditis, Foot rot in humans, Onion bulb rot

Question 19

2nd most common respiratory infection in CF

Answer 19

B. cepacia

Question 20

MaC = Purple Colony
Oxidizer
grows @42 deg celsius

Motility -
Oxidase -
Catalase (+)
LDC -

Answer 20

Acinetobacter baumanni

Question 21

Acitenobacter spp. that is NON OXIDIZER
ASSACHAROLYTIC

Answer 21

Acitenobacter iwoffi

Question 22

(+) Motility
(-) Oxidase
(+) Catalase
(+) LDC

Answer 22

S. MaLtophilia

Question 23

(+) Motility
(+) Oxidase
(+) Catalase
(-) LDC

Answer 23

Pseudomonas

Question 24

Old name: Xanthromonas
LDC (+)
Strong oxidizer of MALTOSE
Lavender green colony
Ammonia like Odor
Causes WOUND INFECTION

Answer 24

Stenotrophomonas maltophilia

Question 25

Urease (-)
Peritrichous
APPLE-LIKE ODOR “FRUITY”

Answer 25

Alkaligenes Faecalis

Question 26

Acinetobacter or Alkaligenes faecalis

(+) OXIDASE
(+) MOTILITY

Answer 26

Alkaligenes faecalis

Question 27

Produces yellow pigment —> Flavin

Answer 27

Chryseobacterium (flavobacterium) meningosepticum