III: Gram-Negative Bacilli (Fermenters) Flashcards
CPOP
Gram-negative comma-shaped, curved
Halophilic
Reduce Nitrate to Nitrite
Most of them are susceptible to O/129
Most are positive to String Test
Vibrio
Vibrio are halophilic EXCEPT:
V. cholerae
V. mimicus
Vibrio has what type of flagella
Peritrichous
Rapid Darting Motility / Shooting Star
Vibrio
A vibrio spp. characterized with RICE WATERY STOOL
V. cholerae
V. cholerae are cultured in
Alkaline Peptone Water
TCBS
In TCBS, Sucrose fermenter will have
Yellow colonies
All Vibrio are NSF except for:
V. cholerae
V. alginolyticus
It is a toxin that increases cAMP causing to have excessive secretion of Water and Electrolytes
Cholera toxin (choleragen)
Phenomenon by which there is a lysis of V.cholerae after injecting immunized host
Pfeiffer’s phenomenon
V. cholera Serogroups
Inaba - Philippines
Ogawa - India
Hikojima - Japan
V. cholera Biotypes (2)
Classical
El Tor
V.cholera biotype that has
Red cell Hemolysis (+)
VP (+)
*Polymyxin B (R)
Agglutination Chix RBC (+)
El Tor
V.cholera biotype that has
Red cell Hemolysis (-)
VP (-)
Polymyxin B (S)
Agglutination Chix RBC (-)
Classical
V. cholera subgroup associated with Epidemic and Pandemic Cholera
V. cholerae O1
V. cholera O139
strains that phenotypically V. cholerae O1 but does not agglutinate in 01 antisera
V. cholerae non-O1
Vibrio Test that uses O/129 (150 ug) impregnated disk
Vibriostatic Test
Vibrio spp that test (+) Vibriostatic
V. cholerae
Plesiomonas shigelloides
Vibriostatic Test (-)
Aeromonas
C. violaceum
String Test Reagent
0.5% Sodium Desoxycholate
Test that differentiates Aeromonas & Plesiomonas from Vibrio
String Test
String Test (+)
Viscous String; Long & Tenacious
Halophilic
Wound infection
Ear Infection
Acquired from Marine Enviroment
Sucrose Fermenter
V. alginolyticus
Vibrio causes GASTROENTERITIS from ingesting contaminated seafoods
Halophilic
NSF
V. parahaemolyticus
causes Summer’s diarrhea
V. parahaemolyticus
Phenomenon of V. parahaemolyticus by which there is a lysis of Human RBC in wagutsuna agar due to Heat Stable Hemolysin
Kanagawa Phenomenon
causes gastroenteritis and Ear infection
Non-halophilic
NSF
V. mimicus
Only Vibrio that is Lactose Fermenters
Causes Septicemia and wound Infection
Halophilic
V. vulnificus
Urease Negative
Gram-negative rods
Beta-hemolytic in BAP
Pink-centered colonies in CIN
Ferments Lactose
Aeromonas
Most common isolate of Aeromonas
Aeromonas hydrophilia
Pink-eye colonies are seen in CIN agar. The patient ingested freshwater foods.
Aeromonas
Straight gram rods; Filamentous forms
gamma-hemolytic in SBA
White to Pink in Inositol Brilliant Green Bile Salt Agar
POSITIVE TRIO - LDC, ODC, ADC (+)
Plesiomonas shigelloides
Slightly curved gram (-), rounded ends
colonies appear black to dark purple
smells like AMMONIUM CYANIDE in SBA
NLF in Macconkey
Violet Pigment can interfere with oxidase test
Chromobacterium viocelium
It is added for Quarum testing
C. vioceleum
Test keys to differentiate Aeromonas & Plesiomonas from Vibrio
Sodium Requirement
Mannitol Requirement
String Test (+)
Vibrio cholerae
Vibrio
TCBS (+)
Vibrio cholerae
Other vibrio
O/129 (+)
V. cholerae
Plesiomonas shigelloides
broth w/o 6.5% NaCl (-)
Other Vibrio
Broth w/ 6.5 NaCl (+)
V. cholerae
Other vibrio
Inositol Fermentation(+)
Plesiomonas shigelloides
LDC +
ODC +
ADC +
Plesiomonas shigelloides
Mannitol fermentation (+)
V. cholerae
Aeromonas
Microaerophilic and Capnophilic
Associated with Gastritis and Diarrhea
Optimum growth 42-43 deg C
Single Polar = Darting Motility
Campylobacter
Growth at 42-43
CHAP
C. jejuni
H.pylori
Acitenobacter baumanni
P. aeruginosa
Culture Medium of Campylobacter
Campy-BAP
Skirrow’s Medium
S-shaped, seagull wings; Lipotrichous Flag
Natural Habitat in stomach
Culture in Biopsies/Urea Breath Test
H. pylori
Usually associated with Peptic Ulcer
H. pylori
Hippurate Hydrolysis (+)
C. jejuni
growth @42
C. jejuni
H. pylori
Growth @25
c. fetus
Urease (+)
H. pylori
Nalidixic acid (S)
C. jejuni
Cephalotin (R)
C. jejuni