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BACTERIOLOGY > III: Gram-Negative Bacilli (Fermenters) > Flashcards

III: Gram-Negative Bacilli (Fermenters) Flashcards

1
Q

CPOP
Gram-negative comma-shaped, curved
Halophilic
Reduce Nitrate to Nitrite
Most of them are susceptible to O/129
Most are positive to String Test

A

Vibrio

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2
Q

Vibrio are halophilic EXCEPT:

A

V. cholerae
V. mimicus

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3
Q

Vibrio has what type of flagella

A

Peritrichous

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4
Q

Rapid Darting Motility / Shooting Star

A

Vibrio

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5
Q

A vibrio spp. characterized with RICE WATERY STOOL

A

V. cholerae

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6
Q

V. cholerae are cultured in

A

Alkaline Peptone Water
TCBS

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7
Q

In TCBS, Sucrose fermenter will have

A

Yellow colonies

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8
Q

All Vibrio are NSF except for:

A

V. cholerae
V. alginolyticus

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9
Q

It is a toxin that increases cAMP causing to have excessive secretion of Water and Electrolytes

A

Cholera toxin (choleragen)

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10
Q

Phenomenon by which there is a lysis of V.cholerae after injecting immunized host

A

Pfeiffer’s phenomenon

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11
Q

V. cholera Serogroups

A

Inaba - Philippines
Ogawa - India
Hikojima - Japan

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12
Q

V. cholera Biotypes (2)

A

Classical
El Tor

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13
Q

V.cholera biotype that has

Red cell Hemolysis (+)
VP (+)
*Polymyxin B (R)
Agglutination Chix RBC (+)

A

El Tor

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14
Q

V.cholera biotype that has

Red cell Hemolysis (-)
VP (-)
Polymyxin B (S)
Agglutination Chix RBC (-)

A

Classical

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15
Q

V. cholera subgroup associated with Epidemic and Pandemic Cholera

A

V. cholerae O1
V. cholera O139

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16
Q

strains that phenotypically V. cholerae O1 but does not agglutinate in 01 antisera

A

V. cholerae non-O1

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17
Q

Vibrio Test that uses O/129 (150 ug) impregnated disk

A

Vibriostatic Test

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18
Q

Vibrio spp that test (+) Vibriostatic

A

V. cholerae
Plesiomonas shigelloides

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19
Q

Vibriostatic Test (-)

A

Aeromonas
C. violaceum

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20
Q

String Test Reagent

A

0.5% Sodium Desoxycholate

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21
Q

Test that differentiates Aeromonas & Plesiomonas from Vibrio

A

String Test

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22
Q

String Test (+)

A

Viscous String; Long & Tenacious

23
Q

Halophilic
Wound infection
Ear Infection
Acquired from Marine Enviroment
Sucrose Fermenter

A

V. alginolyticus

24
Q

Vibrio causes GASTROENTERITIS from ingesting contaminated seafoods

Halophilic
NSF

A

V. parahaemolyticus

25
Q

causes Summer’s diarrhea

A

V. parahaemolyticus

26
Q

Phenomenon of V. parahaemolyticus by which there is a lysis of Human RBC in wagutsuna agar due to Heat Stable Hemolysin

A

Kanagawa Phenomenon

27
Q

causes gastroenteritis and Ear infection

Non-halophilic
NSF

A

V. mimicus

28
Q

Only Vibrio that is Lactose Fermenters

Causes Septicemia and wound Infection

Halophilic

A

V. vulnificus

29
Q

Urease Negative
Gram-negative rods
Beta-hemolytic in BAP
Pink-centered colonies in CIN
Ferments Lactose

A

Aeromonas

30
Q

Most common isolate of Aeromonas

A

Aeromonas hydrophilia

31
Q

Pink-eye colonies are seen in CIN agar. The patient ingested freshwater foods.

A

Aeromonas

32
Q

Straight gram rods; Filamentous forms

gamma-hemolytic in SBA

White to Pink in Inositol Brilliant Green Bile Salt Agar

POSITIVE TRIO - LDC, ODC, ADC (+)

A

Plesiomonas shigelloides

33
Q

Slightly curved gram (-), rounded ends
colonies appear black to dark purple
smells like AMMONIUM CYANIDE in SBA
NLF in Macconkey
Violet Pigment can interfere with oxidase test

A

Chromobacterium viocelium

34
Q

It is added for Quarum testing

A

C. vioceleum

35
Q

Test keys to differentiate Aeromonas & Plesiomonas from Vibrio

A

Sodium Requirement
Mannitol Requirement

36
Q

String Test (+)

A

Vibrio cholerae
Vibrio

37
Q

TCBS (+)

A

Vibrio cholerae
Other vibrio

38
Q

O/129 (+)

A

V. cholerae
Plesiomonas shigelloides

39
Q

broth w/o 6.5% NaCl (-)

A

Other Vibrio

40
Q

Broth w/ 6.5 NaCl (+)

A

V. cholerae
Other vibrio

41
Q

Inositol Fermentation(+)

A

Plesiomonas shigelloides

42
Q

LDC +
ODC +
ADC +

A

Plesiomonas shigelloides

43
Q

Mannitol fermentation (+)

A

V. cholerae
Aeromonas

44
Q

Microaerophilic and Capnophilic
Associated with Gastritis and Diarrhea
Optimum growth 42-43 deg C
Single Polar = Darting Motility

A

Campylobacter

45
Q

Growth at 42-43
CHAP

A

C. jejuni
H.pylori
Acitenobacter baumanni
P. aeruginosa

46
Q

Culture Medium of Campylobacter

A

Campy-BAP
Skirrow’s Medium

47
Q

S-shaped, seagull wings; Lipotrichous Flag
Natural Habitat in stomach
Culture in Biopsies/Urea Breath Test

A

H. pylori

48
Q

Usually associated with Peptic Ulcer

A

H. pylori

49
Q

Hippurate Hydrolysis (+)

A

C. jejuni

50
Q

growth @42

A

C. jejuni
H. pylori

51
Q

Growth @25

A

c. fetus

52
Q

Urease (+)

A

H. pylori

53
Q

Nalidixic acid (S)

A

C. jejuni

54
Q

Cephalotin (R)

A

C. jejuni

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