II: Gram Positive Bacilli

Question 1

Major source: contaminated food
Causes: Granulomatosis Infaseptica

Answer 1

L. monocytogenes

Question 2

L. monocytogenes

Medium
Virulence Test

Answer 2

Mcbrides Medium

Anton’s Test

Question 3

L. monocytogenes

Hanging Drop:
SIM:

Answer 3

Hanging Drop: Tumbling motility
SIM: Inverted X-mas tree/Umbrella

Question 4

L. monocytogenes virulence factor

Answer 4

Listeriolysin O (exit phagosome)
Actin Rackets (stingshot)

Question 5

ONLY GRAM POSITIVE BACILLI THAT IS H2S (+)

Answer 5

Erysipelothrix rhusiopathiae

Question 6

Gelatin Swab: Test tube Brush Pattern

Causes:
ERISIPELOID/ BUTCHERS CUT/ DIAMOND CUT

Answer 6

Erysipelothrix rhusiopathiae

Question 7

Gram (+) Bacilli

Reverse Camp (+)

Exhibit: Phospholipase D (inhibit B-lysin)

Answer 7

Arcanobacterium haemolyticum

Question 8

Gram (+) Bacillus

GI: Boas Oppler’s Bacillus
Vagina: Dodderlain’s bacillus

Answer 8

Lactobacillus acidophilus

Question 9

L. acidophilus grows on

Answer 9

TOMATO JUICE AGAR

Question 10

SPS Positive
causes: Gardnerella vaginosis

Answer 10

Gardnerella Vaginalis

Question 11

G. vaginalis Diagnostic test

Answer 11

1. Amsel and Nugent scoring system
2. Whiff/Sniff Test (Fish-amine odor)

Question 12

Clue cells, foul smelling

Answer 12

G. vaginalis

Question 13

Koch bacillus
Gram ghost/Gram neutral

Virulence factor:
Cord factor & Sulfatides

Answer 13

Mycobacterium tuberculosis