Part II: Gram Positive Cocci (CoNS) and Micrococci Flashcards
All test that are positive in S. aureus are NEGATIVE IN CoNS EXCEPT
Catalase Test
Coagulase Negative Gram (+) Cocci are (2)
S. epidermidis
S. saphropyticus
S. epidermidis
Catalase
Positive
S. epidermidis
Coagulase
Negative
S. epidermidis
Dnase Test
Negative
S. epidermidis
Manitol fermentation test
Negative
S. epidermidis
Phosphatase Test
positive
S. epidermidis
Gelatinase Test
Positive
S. epidermidis
Hemolysis
Gamma
S. epidermidis
Novobiocin Susceptibility Test
Susceptible
S. epidermidis
Clinical Significance
Heart valve infarction
Stitch Abcess
Urinary Tract Infection (UTI
S. saprophyticus
Catalase Test
Positive
S. saprophyticus
Coagulase Test
Negative
S. saprophyticus
Dnase Test
Negative
S. saprophyticus
Mannitol Fermentation Test
Negative
S. saprophyticus
Hemolysis
Gamma
S. saprophyticus
Novobiocin Test
Resistant
S. saprophyticus
Phosphatase Test
Negative
S. saprophyticus
Gelatinase Test
Positive
S. saprophyticus
Clinical Significance
Most common cause of UTI in young women
- Pyelonephritis
- Cystitis
S. epidermidis
Virulence factor
Slime production / Biofilm formation
enhances ability of organisms to
attach/adhere to inorganic surfaces (plastic catheters); inhibit/prevent penetration of antibiotics
Slime/Biofilm
Gram (+) cocci in tetrads/cuboidal packets
Micrococci
Micrococci
a. Strict anaerobe
b. Strict aerobe
strict aerobe
Micrococci : Catalase Test
Positive
Micrococci : Hemolysis
Gamma
Presumptive Test for Genus Micrococci
Modified Oxidase Test / Microoxidase
Modified Oxidase Test / Microoxidase
6% Oxidase
Modified Oxidase Test / Microoxidase (+) Result
BLUE/PURPLE-BLUE COLOR
(Ferment glucose)
Staphylococci
(Oxidize glucose)
Micrococci
Staphylococci : Aerobic Growth
+
Staphylococci : ANAEROBIC
GROWTH
+
Staphylococci : LYSOSTAPHIN
SUSCEPTIBILITY
Susceptible
Staphylococci : BACITRACIN
SUSCEPTIBILITY
Resistant
Staphylococci : FURAZOLIDONE
/ FUROXONE
SUSCEPTIBILITY
Susceptible
Staphylococci : CATALASE TEST
+
Staphylococci : MODIFIED
OXIDASE TEST
-
BENZIDINE TEST
-
can produce acid aerobically
and anaerobically;
Ferment glucose
can produce acid only in the
presence of air
Oxidize glucose
Micrococci : AEROBIC
GROWTH
+
Micrococci : ANAEROBIC GROWTH
-
Micrococci : LYSOSTAPHIN SUSCEPTIBILITY
Resistant
Micrococci : MODIFIED OXIDASE TEST
Positive
Micrococci : BACITRACIN SUSCEPTIBILITY
Susceptible
Micrococci : FURAZOLIDONE / FUROXONE
SUSCEPTIBILITY
Resistant
Micrococci : CATALASE TEST
+
Micrococci : BENZIDINE TEST
+