Haemophilus

Question 1

Non- motile
Non-sporeforming
Facultative Anaerobe
CPOP
Obligate Parasites
Requires Growth Factors

Answer 1

Haemophilus

Question 2

All haemophilus are fermenters except:

Answer 2

H. ducreyi

Question 3

Growth factors for Haemophilus

Answer 3

X factor - hemin
V factor - NAD

Question 4

Requires X and V factor

Answer 4

H. influenzae
H. haemolyticus
H. paraphrophilus

Question 5

Requires V factor

Answer 5

H. parainfluenzae
H. parahaemolyticus
H. paraphrophilus

Question 6

Requires X factor

Answer 6

H. ducreyi

Question 7

Beta-hemolytic haemophilus

Answer 7

H. haemolyticus
H. parahaemolyticus

Question 8

D-ALA (+)

Answer 8

H. parainfluenzae
H. parahemolyticus
H. parahrophilus
H. aphrophilus

Question 9

Preferred incubation:

5-10% CO2
35-37 deg celsius
24-72 hrs

Answer 9

Most Haemophilus spp

Question 10

Preferred incubation

5-10% CO2
33 deg celsius
7 days

Answer 10

H. ducreyi

Question 11

Specimen processing and Isolation for Haemophilis

Answer 11

Pre-moistened swab
Stuart and Amies

Question 12

Culture Media used for Haemophilus

Answer 12

1. Chocolate Agar with Bacitracin
2. Chocolate Agar with 1% IsoVitaleX (H.decreyi and H. aegypticus)
3. Nairobi Biiplate Medium

Question 13

Laboratory Identification for Haemophilus

Answer 13

1. Neufeld Quellung’s Test
2. Staphylococcus Streak
3. X and V factor Requirement and Hemolytic Pattern (Using Horse blood)
4. Porphyrin Test

Question 14

Laboratory Identification for Haemophilus:

Use of Antisera; observe for capsular swelling

Answer 14

Neufeld Quellung’s Test

Question 15

Laboratory Identification for Haemophilus

Haemophilus is streaked with S. aureus, S. pneumoniae, Neisseria, or certain yeast

Answer 15

Staphylococcus Streak

Question 16

Laboratory Identification for Haemophilus

(+) for Staphylococcus streak shows

Answer 16

Haemophilus appears “DEWDROP” colonies

Question 17

Laboratory Identification for Haemophilus

Test for the ability of the organism to

ALA —> Phorphobilinogen or porphyrins
Porphyrin –> Hemin

Answer 17

Porphyrin Test

Question 18

Porphobilinogen is detected using

Answer 18

Kovac’s reagent (PDAB)

Question 19

Porphyrin is detected using

Answer 19

Fluorescence (Reddish-orange at UV Light 360 nm)

Question 20

Formerly known as “Pfeiffer’s bacillus”

Answer 20

H. influenzae

Question 21

1. Known as “Pfeiffers bacillus”
2. Has 6 serotypes
3. Causes Meningitis in <5 yrs old
4. associated with RESPIRATORY CONDITIONS AND EPIGLOTTIS
5. XYLOSE POSITIVE

Answer 21

H. influenzae

Question 22

Causes meningitis in <5 yrs old

Answer 22

H. influenzae

Question 23

Formerly known as “Koch Bacillus”

Answer 23

H. aegypticus

Question 24

1. Closely resembles H. influenzae biotype III
2. causes PURULENT CONJUNCTIVITIS / Brazillian Purpuric Fever

Answer 24

H. aegypticus

Question 25

Infective agent of Chancroid (Soft chancre; painful)

Answer 25

H. ducreyi

Question 26

In MS Examination of H. ducreyi it appears

Answer 26

“Schools of Fish” “Trailroad of tracks”
“Fingerprint from genitelia”

Question 27

BIOCHEMICALLY INERT haemophilus spp

Answer 27

H. ducreyi

Question 28

Colony morphology:

Encapsulated
Bleach/Mousy odor in CAP

Answer 28

H. influenzae

Question 29

Colony Morphology:

Can be pushed intact
forms CLUMPS in saline

Answer 29

H. ducreyi