S1 [LAB]: Exp 2: Preparation of Red Cell Suspension; Serial Dilution

Question 1

A red cell suspension is prepared from what specimen?

Answer 1

Anticoagulated whole blood

Question 2

Concentration of RBC in a red cell suspension

Answer 2

2-5%

Question 3

Tonicity of the saline to prepare a red cell suspension

Answer 3

Isotonic

Question 4

The ___ of antibodies in serum to antigens in red cell suspension affect the sensitivity of serologic assays

Answer 4

ratio

Question 5

The process of suspending the red cells in isotonic saline, gently mixing by inversion, centrifugation, and decantation several times

Answer 5

Washing

Question 6

The process of removing all traces of plasma and other non-red cell entities in the whole blood samples

Answer 6

Washing

Question 7

Traces of plasma and other non-red cell entities may affect ___ reactions

Answer 7

Antigen-antibody

Question 8

Added to the concentrate to make the reagent the proper strength for testing

Answer 8

Water or saline

Question 9

Necessary to make a less concentrated solution from a reagent such as an acid or a buffer in order to use the reagent in a particular procedure

Answer 9

Dilution

Question 10

The material being diluted

Answer 10

Solute

Question 11

The medium making up the rest of the solution

Answer 11

Solvent/ Diluent

Question 12

A dilution wherein an initial dilution is made to make succeeding dilutions

Answer 12

Serial dilution

Question 13

A process where there is a small amount of serum serially diluted by transferring aliquot to a fixed amount of diluent

Answer 13

Serial dilution

Question 14

Serial dilution can either be done in ___ or ___

Answer 14

Test tubes
Microtiter plates

Question 15

Reciprocal of the highest dilution of antibody in which it is still detectable

Answer 15

Antibody titer

Question 16

In hemagglutination reaction, this is the reciprocal of the last tube or well with agglutination

Answer 16

Antibody titer

Question 17

Concentration of the red cell suspension needed for the serial dilution

Answer 17

5%

Question 18

Diluent used in serial dilution

Answer 18

Normal saline solution

Question 19

Specimens that may be used for serial dilution

Answer 19

Pooled serum sample
Whole/ anticoagulated blood

Question 20

In the microtiter plate method of serial dilution, the plate is left undisturbed for how long and at what temperature?

Answer 20

30-45 mins
Room temperature

Question 21

T/F: In red cell suspension, an approximation achieves the appropriate serum-to-cell ratio for most test procedures

Answer 21

True

Question 22

An indication of incomplete washing, suggesting that traces of plasma are still present

Answer 22

Yellow appearance

Question 23

An indication that hemolysis has occurred

Answer 23

Red supernatant fluid

Question 24

Defined as the volume of a sample per total volume

Answer 24

Dilution

Question 25

A series of half dilutions wherein each successive tube will have half the amount of the original concentrated solution

Answer 25

Doubling dilution/ Twofold dilution