2S [LAB]: C3 Flashcards
Detect and measure the complement proteins in a clinical sample as antigens
Antigenic assays
Simple, rapid, easy-to-perform, and convenient for routine testing
Antigenic assays
Representative method of antigenic assays
Radial immunodiffusion technique
Assess the functions of different complement proteins
Functional assays
Sensitive, provides accurate information about the activity of a complement component
Functional assays
A diagnostic test intended for the identification of antigens or for the quantitative study of antibodies
Immunodiffusion
Constitutes 70% of the total proteins in the complement system
C3
C3 is measured in the lab as a routine practice, alongside ___ and ___
C1q and C4
Useful in the monitoring inflammatory conditions such as nephritis and SLE
C3
The determination of human C3 uses what type of assay, intended for the quantification of the serum C3 complement protein?
Antigen detection assay (radial immunodiffusion method)
The Mancini method of RID is observed until how many hours?
48 hours (endpoint)
The Fahel & McKelvey method of RID is observed until how many hours?
17-24 hours
In the preparation of RID plate, the refrigerated sealed pouch of the RID plate to reach room temperature by letting it stand for approximately how many minutes?
30 mins
To determine the titer in the functional assay, what reagent is used?
50% hemolyzed RBCs
In using the RID test kit, how much sample is dispensed on the designated wells?
5 uL
Unit used to measure C3 value
md/dL
A serologic reaction in which particulate antigens suspended in fluid form clumps or aggregate in response to the presence of specific antibodies
Agglutination
A type of agglutination that uses a known antiserum to identify unknown antigens intrinsic in clinical samples or from unknown suspensions of microorganisms
Direct agglutination
A type of agglutination intended to detect antibodies in a person’s serum with the use of a known antigen
Indirect/ passive agglutination
Utilizes a known antigen that coats onto latex or other inert particles to identify antibodies on a patient’s serum
Classic passive agglutination
The antibody, rather than the antigen, is covalently linked to the carrier particle and mixed with a patient’s serum in order to detect the presence of the unknown antigen or other immune factors
Reverse passive agglutination
A modified type of agglutination reaction that is highly sensitive for identifying small amounts of antigens in a patient’s sample
Agglutination inhibition
A method used for the epidemiologic studies of some viral diseases, home-based pregnancy test, and, in some countries, as a premarital test to determine the immune status of females against rubella infection
Agglutination inhibition
What is integrated to the RID agarose gel?
Anti-c3c
A classic method of detecting antigen-specific antibodies
Complement fixation
Positive result for complement fixation
Absence of red cell lysis
Negative result for complement fixation
Presence of hemolysis
A method where specific antibodies, as they bind with their corresponding antigens, are able to block or nullify the chemical activity or biologic effects of the antigen
Neutralization
Positive result for neutralization
Absence of hemolysis
Negative result for neutralization
Presence of hemolysis
