S1 [LAB]: Exp 16: Nitroblue Tetrazolium Test Flashcards
This screening test is useful in differentiating bacterial from non bacterial infections and has been used in diagnosing chronic granulomatous disease (CGD)
Nitroblue tetrazolium test
Group of disorders inherited as either an X-linked or autosomal recessive gene that affects neutrophil microbicidal function
Chronic granulomatous disease
In CGD, infections usually begin at what age?
1 year old
Most common and best describe of the neutrophil abnormalities
Chronic granulomatous disease
NBT reduction is caused by the production of ___ and ___
Hydrogen peroxide
other reactive forms of oxygen
Reduction converts the nearly colorless NBT into ___ that can be assessed visually on a microscope slide
blue precipitate
The fixative used in nitroblue tetrazolium test
Methanol
The stain used in nitroblue tetrazolium test
Wright stain
In the NBT assay, there is a ___ times increase if there is a bacterial infection
5-7
If neutrophils kills less than ___ of bacteria, one may consider defect in intracellular killing
10%
