Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

MT6326 > 2S [LAB]: Serologic Tests (secondary) > Flashcards

2S [LAB]: Serologic Tests (secondary) Flashcards

1
Q

Principle of TPHA

A

Indirect Hemagglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Carrier particles used in TPHA

A

Red cells (Avian, but Bovine may also be used)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Non-virulent strain of Treponema used in TPHA

A

Reiter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Pathogenic strain of Treponema

A

Nichol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

In TPHA, the setups are mixed for how long and is left undisturbed for how long?

A

2 mins
45 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Principle of VDRL

A

Flocculation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Aggregation of non-cellular particulate matter

A

Flocculation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

IgM isotype formed after interaction of phospholipid from spirochetes interacting with immune system of the host

A

Non-treponemal antibodies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Principle of RPR

A

Flocculation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Source of antigen used in RPR

A

Cardiolipin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Alcoholic extract of beef’s heart

A

Cardiolipin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Positive result of RPR

A

Black aggregates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Negative control of VDRL

A

NSS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Rotation condition when doing VDRL

A

180 rpm for 4 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Rotation condition when doing RPR

A

100 rpm for 8 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Widal test is used in which infection?

A

Salmonella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Weil-Felix test is used in which infection?

A

Rickettsia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Antigen detected in Widal test

A

Somatic flagellar antigen (from Salmonella)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Delayed procedure in Weil-Felix test causes what effect?

A

Fake agglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Interpret:

OX-19 (+)
OX-2 (Weak)

A

Typhus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Interpret:

OX-19 (-)
OX-2 (-)
OX-K (+)

A

Tsutsugamushi fever/ disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

In Brucella test, a titer above ___ denotes an active infection

A

1/80

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Etiologic agent of syphilis

A

Treponema pallidum subsp. pallidum

24
Q

Etiologic agent of Yaws

A

T. pallidum subsp. pertenue

25
Q

Etiologic agent of non-venereal disease

A

T. pallidum subsp. endemicum

26
Q

Motility of T. pallidum

27
Q

Also known as the “great imitator”, French disease, Italian disease

28
Q

Lesions present in primary syphilis

A

Hard chancre

29
Q

Body parts affected by the chancre

A

Glans penis (male)
Labia (female)

30
Q

Lesions present n secondary syphilis

A

Condylomata lata

31
Q

Phase in syphilis infection mistaken as recovery

A

Latent phase

32
Q

Degeneration of lower spinal cord that is a common manifestation of tertiary syphilis

A

Tabes dorsalis

33
Q

Lesions present in tertiary syphilis

34
Q

Microscope used in the direct detection of syphilis

A

Dark field

35
Q

The direct method of detection of syphilis is only advisable for which phase?

A

Primary/ secondary

36
Q

Phospholipid released that will react to the immune system of the host following tissue destruction

37
Q

The main antigen in VDRL

A

Cardiolipin

38
Q

Reagent in VDRL that enhances reagin reactivity

A

Cholesterol

39
Q

Reagent in VDRL that removes anti-complementary substances of cardiolipin

40
Q

Qualitative serum VDRL uses what size of needle? How much of the Ag suspension per mL will be delivered?

A

18 g
60 drops

41
Q

Quantitative serum VDRL uses what size of needle? How much of the Ag suspension per mL will be delivered?

A

19 g
75 drops

42
Q

Conditions of the water bath used in VDRL

A

56C for 30 mins

43
Q

VDRL is done at what temperature?

44
Q

The VDRL result is observed under what magnification?

45
Q

RPR reagent that makes the reaction more visible

46
Q

RPR reagent that prevents lipid peroxidation

47
Q

RPR reagent that preserves the suspension

A

Thimerosal

48
Q

RPR reagent that acts as a chemical inactivator

A

Choline chloride

49
Q

Source of live treponeme antigens used in the T. pallidum immobilization test (TPI)

A

Rabbit testes

50
Q

Causative agent of Lyme disease

A

Borrelia burgdorferi

51
Q

Titer that is usually considered as positive for lyme disease screening

52
Q

Antigen used in TPHA

A

Avian (turkey) RBCs fixed w/ glutaraldehyde and tannic acid

53
Q

The control cell in TPHA are coated with ___

A

Reiter’s strain

54
Q

The test cell in TPHA are coated with ___

A

Nichol’s strain

55
Q

The Ag suspension in VDRL appears to be ___

A

Milky white

MT6326 (24 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions