2S [LAB]: Secondary Serologic Tests Flashcards
Scientist who proposed the lattice hypothesis
Marrack
“Phase change reaction”
Precipitation
Serologic reaction that involves the interaction of a soluble Ag and an Ab
Precipitation
Antibody most commonly considered as precipitin
IgG
Method of precipitation detection:
Light scattering
Nephelometry
Method of precipitation detection:
Light transmitted
Turbidimetry
Medium used in precipitation reaction
1% Agarose gel (semi-solid medium)
Diffusion of Ag and/or Ab until it reaches the correct Ag-Ab proportion and then precipitates out of solution
Immunodiffusion
A single linear ID wherein known Ab is incorporated with gel is allowed to solidify. In this method, the sample is added by overlaying on top of solidified agar
Oudin test
A method of quantification of Ag by measurement of diameter of precipitation ring; samples are placed in wells
Single radial ID
In Single radial ID, agarose plate is coated with ___
Known Ab
In Single radial ID, the end point method is the ___
Mancini
In Single radial ID, the kinetic method is ___
Fahey McKelvey
A double ID method where wells are arranged at the apices of an imaginary equilateral triangle
Ouchterlony or Double Angular ID
A double ID method of determination of relationships of antigens in respect to Ab
Ouchterlony or Double Angular ID
A one-stage technique of single ID which measures the height of rockey
Laurell/ Rocket Immunoelectrophoresis
A technique where both Ag and Ab diffuse through an agarose gel until they meet at a point of equivalence
Double Simple ID
Immunofixation electrophoresis was first described by ___
Alper and Johnson
Immunofixation electrophoresis method where the antisera (Ab) is applied directly on the gel rather than placed in a through
Immunoprecipitation
Western, Southern, and Northern blotting are all examples of ___
Immunofixation electrophoresis
Serologic reactions involving particulate or insoluble Ag
Agglutination
Antibody most often considered as an agglutinin
IgM
Agglutination reaction where the Ag is inherently present on cell surface
Direct agglutination
Agglutination reaction applied on ABO typing
Direct agglutination
Agglutination reaction applied on Widal Test
Direct agglutination
Agglutination reaction where the Ag is coated to carrier particle
Passive agglutination
Agglutination reaction where the Ab is coated to carrier particle
Reverse passive agglutination
T/F: The positive result for reverse passive agglutination is the absence of agglutination
False
Antiglobulin test that utilizes in vivo sensitization of RBCs
Direct Coombs/ Direct Antihuman Globulin Test
Antiglobulin test that utilizes in vitro sensitization of RBCs
Indirect Coombs/ Indirect Antihuman Globulin Test
Antibody utilized in the AHG reagent
IgG
Agglutination reaction that uses Staphylococcus aureus as carrier of Ab
Coagglutination
Positive result for complement fixation
No hemolysis
Reaction in which the antigenic activity is stopped/ nullified by a specific Ab
Neutralization
Principle of ASO tube test
Toxin Neutralization
Principle of Schick’s Test
Toxin Neutralization
Principle of Dick’s Test
Toxin Neutralization
Also known as natural clumping
Flocculation
A reaction that causes aggregation of colloidal particles and production of fleecy mass
Flocculation
Midway reaction between agglutination and precipitation
Flocculation
Principle of VDRL test
Flocculation
Non-cellular particulate Ag
Flocculation
