ROS

Question 1

Where is hydrogen peroxide made?

Answer 1

Peroxisomes, RER, mitochondria

Question 2

What enzyme catalyzes the reaction of hydrogen peroxide (H2O2) to hypochlorous acid (HOCl)?

Answer 2

Myeloperoxidase

Question 3

In what cells is myeloperoxidase most active?

Answer 3

Phagocytes, neutrophils especially, during the oxidative burst phase of phagocytosis

Question 4

Reactive nitrogen oxygen species all stem from what chemical compound?

Answer 4

Nitric oxide, NO, made by nitric oxide synthase from the transformation of arginine to citrulline

Question 5

What is the causative genetic defect in chronic granulomatous disease and in what cells is this most detrimental?

Answer 5

NADPH oxidase in phagocytes

Question 6

What is the problem in CGD?

Answer 6

Not enough hydrogen peroxide is made to destroy catalase positive bacteria (ex. Aspergillosis, S. Aureus, Serratia, Nocardia)

Question 7

What is the most effective diagnostic test for CGD?

Answer 7

Nitro blue tetrazolium test

Question 8

What medications will a CGD patient need to take for the rest of their life?

Answer 8

Itraconazole and bactrim

Question 9

What is ischemia reperfusion injury?

Answer 9

.