B and T Cell Maturation Flashcards
TF GATA-3 promotes development of what cells?
TH2
Name the stages of progression for B cell development?
early pro-B, late Pro-B, large pre-B, small pre-B, immature, transitional 1, transitional 2, mature
What is one disadvantage of having TI mediated B cell activation without T helper involvement?
no memory
What does c-Kit do?
regulates replication
Double negative (DN) 2 thymocytes are positive for these genes?
c-Kit, CD 44 and 25
Which transcription factor promotes development of TH1?
T-BET
What cytokines are required for B cell differentiation?
IL-3, 4 and LBCGF (low molecular weight B cell growth factor)
What is the life-span for a naive B cell?
days to weeks
Define anergy in context of T cell development
a T cell is not costimulated by B7 and is not activated
Where does negative selection occur for B cells?
bone marrow
Pre-B lymphocytes have this receptor to promote proliferation and division?
IL-7R
If a thymocyte is positive for CD 44, what instruction is it receiving?
to relocate to the thymus
All double negative thymocytes express this gene?
c-Kit
Double negative thymocytes 2,3,and 4 (T cells DN2, DN3, and DN4) are found in what location?
sub-scapular cortex of thymus
What are some TH2 cytokines that promote B cell division? That promote B cell differentiation?
division= IL-4 differentiation= IL-4, 5, 6, 10, 13
Where do you find B1 cells?
peritoneum and pleural cavities
Low serum Ig memory cells, class switch recombination and B cell activation are all symptoms of what deficiency and what is the cause?
common variable immunodeficiency, mutation in CD 40, 19 BCR, or a co-stimulating molecule
Binding of what protein to the BR3 receptor of a B cell in transitional phase promotes what?
protein= Blys promotes=survival
Describe a super-antigen. Where does it bind? Where is it found?
binds CD28 , found in staphylococcal infections (food poisoning)
What markers and immunoglobins would you expect to find on a B2/follicular cell?
CD 19/21, 23, IgM and IgD
B cells an be activated without T helper involvement via these antigens? (name two types)
T independent (TI) 1 = bacterial cell wall components like LPS (binds TLR4) T independent (TI) 2= large polysaccharides like flagellin or viruses that bind BCR
A mutation in the Bruton tyrosine kinase results in what deficiency?
agammaglobulinemia = immunodeficiency XLA
Double negative thymocyte 4 (DN4) mature to what T cell stage?
DP (double positive) T cells
How often do B cells in the marginal zone of the spleen self-renew?
long-lived (no self renewal necessary)
Compare the lag-time of a memory B cell to that of a naive B cell.
memory= 1-3 days naive= 4-7 days
