protein lecture 3 Flashcards
what is the Gibbs free energy?
maximum energy of a reaction
what is an enthalpy?
internal energy
what is an entropy?
a disorder
what is it when the energy proceeds?
exergonic
what is it when the energy is reversed?
endergonic
if a reaction is endergonic the change in Gibbs free energy is?
positive
what are the examples of the standard state conditions?
reactants and products at 1 molar concentrations
pressure of all gases at 1 atmosphere
temperature is 25c
PH7
under the standard state conditions what temperature are the reactions performed?
25
if a reaction is at the equilibrium what is the Gibbs free energy?
zero
how can Keq be described as?
products/ reactants
what happens in a reaction coupling?
the exergonic reaction drive endergonic reaction.
what is ATP hydrolysis?
exergonic
what is included in the ATPase Assay?
ATP hydrolysis
2x enzymes : pyruvate kinase and lactate dehydrogenase
3x reagents: ATP, phosphoenolpyruvate and NADH
what reaction is the ATP synthesis?
endergonic
what happens when they have a more positive E0?
stronger oxidising agent - electron acceptor
weaker reducing agent
what happens when they is more negative E0 ?
weaker oxidizing agent
stronger reducing agent- electron donor.
what does the ETC promote?
H+ gradient across the inner mitochondrial inner membrane
what does RCC1 uses?
NADH
what does RCCII uses?
FADH2
how much ATP/NADH produced from RCCI?
2.5
how much ATP/FADH2 is produced from RCCII?
1.5
what is the ratio in the bioenergetics?
NADH and FADH2 - Phosphate to Oxygen Ratio (P:O)
what is included and produced in the RCCI ?
- Complex I (Pumps 4 x H+)
- Complex III (Pumps 2 x H+)
- Complex IV (Pumps 4 x H+) = 10 x H
What is included and produced in the RCCII?
Complex II (Not a pump)
- Complex III (Pumps 2 x H+)
- Complex IV (Pumps 4 x H+) = 6 x H+
