Perio Articles Flashcards
In Davis’s 2016 review “Gene Sequence Analyses of the Healthy Oral Microbiome in Humans and Companion Animals: A Comparative Review,” what 6 phyla dominated the oral communities of humans, dogs and cats?
JVD 2016, Issue 2
Bacteroidetes
Proteobacteria
Firmicutes
Actinobacteria
Fusobacteria
Spirochaetae
In Davis’s 2016 oral biome review article, the healthy oral microbiome in people is dominated by what type of bacteria?
JVD 2016, Issue 2
Gram-positive bacteria belonging to genus Streptococcus
In Davis’s 2016 oral biome review article, the healthy oral microbiome in dogs is dominated by what type of bacteria?
Gram negative spp Moraxella, Bergeyella and Neisseria
The bacterial culture-independent studies of dog oral microbiota from Segata 2012, Dewhirst 2012 and Davis 2013 found which bacterial phyla to most predominate?
Firmicutes most predominate
Segata and Dewhirst - Proteobacteria second most predominate
Davis - Bacteroidetes second most predominate
According to Davis’s 2016 review article, what are the top 3 most predominate oral bacterial phyla in dogs?
Davis. Gene Sequence Analyses of the Healthy Oral Microbiome in Humans and Companion Animals: A Comparative Review. JVD 2016, Issue 2.
Firmicutes
Proteobacteria
Bacteroidetes
According to Davis’s 2016 review article, what are the 4th-6th most predominate oral bacterial phyla in dogs?
Actinobacteria
Fusobacteria
Spriochaetes
According to Perez-Salcedo’s 2013 paper “Isolation and identification of Porphyromonas ssp. and other putative pathogens from cats with periodontal disease,” what bacterial spp was found in 90% of cats with varying stages of PD?
JVD 2014, issue 4
Fusobacterium nucleatum
P. gulae 86%
P. circumdentaria 70%
In Harris’s 2016 paper “A pyrosequencing investigation of differences in the feline subgingival microbiota in health, gingivitis and mild periodontitis,” what percentage of subgingival bacterial species in healthy cats were Gram negative bacteria
75%
In Harris’s 2016 paper “A pyrosequencing investigation of differences in the feline subgingival microbiota in health, gingivitis and mild periodontitis,” what was the relationship between relative percentages of Gram positive and negative bacteria and periodontal health status?
Percentage of gram positive spp increased as periodontal health status declined
Cats with mild periodontitis had equal numbers of Gram positive and Gram negative spp subgingivally
According to Davis’s 2016 review “Gene Sequence Analyses of the Healthy Oral Microbiome in Humans and Companion Animals: A Comparative Review,” dogs and cats have more gram negative or gram positive oral bacteria in health?
Gram negative
Opposite of humans
In Kato’s 2011 study “Molecular Detection of Human Periodontal Pathogens in Oral Swab Specimens from Dogs in Japan,” what was the most common spp of Porphyromonas identified?
JVD 2011, Issue 2
P. gulae
P. gingivalis only identified in 1 dog
In Rawlinson’s 2011 paper “Association of periodontal disease with systemic health indices and systemic response to treatment of periodontal disease,” what indices were associated with periodontal disease?
JAVMA 2011
Attachment loss and platelet number
Decrease in C-reactive protein after treatment correlated with severity of PD
Creatinine moderately negatively correlated with attachment loss
BUN concentration sig increased after treatment of PD
In Kyllar’s 2013 study “Morphometric Assessment of Periodontal Tissues in Relation to Periodontal Disease in Dogs,” what was the relationship between thickness of periodontal tissues and dog size/weight?
JVD 2013, Issue 3
Periodontal tissues were stat sig thinner ( P < 0.001) in Group 1 toy breed dogs (< 5.0kg) than the small, medium sized dog groups
Periodontal tissues were also significantly thicker in areas of canine teeth and carnassial teeth
In Riggio’s 2011 article “Molecular identification of bacteria associated with canine periodontal disease,” what predominant spp were identified for dogs with periodontitis using culture and PCR respectively?
Vet Microbiol 2011
Culture: Actinomyces canis
PCR: Desulfomicrobium orale
In Glickman’s 2011 article “Association between chronic azotemic kidney disease and the severity of periodontal disease in dogs,” what associations were found?
Prev Vet Med 2011
The hazard ratio for azotemic CKD increased with increasing severity of periodontal disease
Increasing severity of periodontal disease also associated with serum creatinine > 1.4 and BUN > 36
What are the main bacteria that produce volatile sulfur compounds?
Millela, JVD 2015, Issue 2
Porphyomonas gingivalis
Prevotella intermedia
Fusobacterium nucleatum
Treponema denticola
What are the two Volatile sulfur compounds (VSCs) most associated with oral malodor?
Millela, JVD 2015, Issue 2
Hydrogen sulfide (H2S)
Methyl mercaptan (CH3SH)
Are the concentrations of hydrogen sulfide and methyl mercaptan higher in the mouth or periodontal pockets respectively
Millela, JVD 2015, Issue 2
Hydrogen sulfide: the mouth
Methyl mercaptain: periodontal pocket
What is the role of volatile sulfur compounds on the development of periodontitis?
MIlella, “The Negative Effects of Volatile Sulphur Compounds,” JVD 2015, Issue 2
Increased permeability of oral mucosa → allows LPS and prostaglandins to penetrate → inflam response → exposure of connective tissue to periodontal pathogenic compounds
Hydrogen sulfide may contribute to alveolar bone resorption via expression of what?
MIlella, “The Negative Effects of Volatile Sulphur Compounds,” JVD 2015, Issue 2
RANKL
In Wallis’s 2016 paper “Quantification of Canine Dental Plaque Using Quantative Light-Induced Fluorescence,” plaque needs to be disclosed or undisclosed when using QLF to measure efficacy of products in reducing total plaque volume?
JVD 2016, Issue 1
Disclosed: not all bacteria in mature biofilms autofluoresce and QLF underestimates amount of total plaque on undisclosed teeth
What were the methods of Riggio’s 2011 Vet Microbiol paper “Molecular identification of bacteria associated with canine periodontal disease?”
JVD 2011, issue 4
Swabs obtained from gingival margin of three dogs with gingivitis and three orally healthy controls and subjected to routine baccterial culture and culture independent methods
First study to use culture-independent methods for identification of bacteria associated with perio in dogs
In Perez-Salcedo’s 2011 Vet Microbiol article “Comparison of two sampling methods for microbiological evaluation of periodontal disease in cats,” what two sampling methods were compared and which was most effective?
JVD 2011, Issue 4
Cottons swabs over mucosa and teeth vs subgingival paper points. Paper points most effective.
paper points had significantly higher recoveries of anaeorobic bacteria and periodontal pathogens
In the 2012 paper on susceptibility of Porphy-romonas spp. and Fusobacterium spp in dogs, what antibiotics showed some resistance patterns?
Senhorinho GN, Nakano V, Liu C, et al. Occurrence and antimicrobial susceptibility of Porphy-romonas spp. and Fusobacterium spp. in dogs with and without periodontitis. Anaerobe. 2012 May 17.
clarithromycin, erythromycin and metronidazole