PCR made simple

Question 1

WHat does pcr stand for

Answer 1

polymerase chain reaction

Question 2

polymerase chain reaction

Answer 2

• amplify a sample of DNA

Question 3

What is in the mixture of PCR

Answer 3

• reaction mesuture set up, contains:
  
  • DNA
  • free nucleotides
  • primers
  • DNA polyerase

Question 4

primer

Answer 4

• short pieces of DNA that are complementary to the bases at the start of the fragment you want

Question 5

DNA polymerase

Answer 5

enzyme that creates new DNA strands

Question 6

what are the strages of PCR

Answer 6

• heated to 95
  
  • break H-bonds
  • DNA polymerase doesnt denature
• mixture cooled to between 50 and 65
  
  • so the primers can bind (anneal) to the strands
• heated to 72
  
  • so DNA polymerase can work
• DNA polymerase lines up free DAN nucleotides alongisde each temp;late strand
  
  • complementary base pairing means new complementary strands are formed
• wo new copies are formed and one cycle of PCR is complete
• repeat, all 4 strands used as templates