(P) Lec 1.2: Microscopy Flashcards
Refers to the study of the human body structures with
the aid of a microscope
Microscopic HSB
Refers to the study of the normal structure and function of
different tissues and organs
Histology
Refers to the study of the structure and function of plant
and animal cells
Cytology
Linear Equivalents
1 Angstrom (Å)
0.1 nanometer (nm)
Linear Equivalents
10 Angstroms
1.0 nanometer
(formerly millimicron
[mu])
Linear Equivalents
1,000 nanometers
1.0 micrometer (um)
(formerly micron U)
Linear Equivalents
1,000 micrometers
1.0 millimeter (mm)`
This is the ability of a microscope lens or optical system to produce separate images of closely positioned objects
Resolving power
Refers to the smallest distance between two particles that can be distinguished from each other
Resolving power
This is the e product of the separate magnification powers of the objective lens and ocular lens
Total magnification of the object
Enumerate the types of microscope (sorry huhu)
- Light
- Electron
- Scanning
- Phase Contrast
- Interference
- Fluorescent
- Polarizing
- Ultraviolet
- Darkfield
- X-Ray
Type of microscope which is simple, compound, and allows visualization of greater detail
Light Microscope
Light microscope goes through which principle?
Refraction of light
Type of microscope that uses electricity?
Electric microscope
Type of Microscope
- Enables examination of unstained cells and tissues
- Especially useful for living cells
Phase Contrast Microscope
Type of Microscope
- Study of mitosis
- Renders chromosomes and
other cell organelles darker than the surrounding cytoplasm.
Phase Contrast Microscope
Type of Microscope
- A modification of phase contrast
- Allows quantification of tissue mass
Interference Microscope
Type of Microscope
- Provides tridimensional images
of living cells and its components.
Interference Microscope
Type of Microscope
- Makes use of the ability of certain molecules to fluoresce under ultraviolet light
Fluorescent Microscope
Type of Microscope
- Useful in localizing
antigen-antibody complexes within
tissues.
Fluorescent Micrsocope
Type of Microscope
- Highly ordered molecules or arrays of molecules can rotate the angle of the plane of polarized light
Polarizing Microscope
Type of Microscope
- Spindle fibers of dividing cells
- Striated muscles
Polarizing Microscope
Type of Microscope
- Uses quartz lenses with an ultraviolet light source
Ultraviolet Microscope
Type of Microscope
- Detects nucleic acids and proteins that contain certain amino acids
Ultraviolet Microscope
Type of Microscope
- No direct light from the light source is gathered by the objective lens
Dark-field Microscope
Type of Microscope
- Urine crystals like UA and oxalate
- Spirochetes
Dark-field Microscope
Type of Microscope
- Combines components of a light optical microscope with a scanning system to dissect a specimen optically
- Permits three dimensional analysis
Scanning MIcroscope
Type of Microscope
- Visualizes a specimen in
very thin sections - Creates extremely sharp image
Scanning Microscope
Type of Microscope
- Greater penetration
- Higher resolving power
X-Ray Microscope
This microscope uses the interaction of a beam of electrons with
a specimen to produce an image
Electron Microscope
This microscope has beams emitted by tungstun filaments and has a higher resolution
Electron Microscope
