Osmotic resistance of erythrocytes

Question 1

What is osmotic resistance of RBC?

Answer 1

a concentration interval (resistance interval) without any haemolytic effect.

Question 2

What is haemolysis?

Answer 2

When the intracellular mass is released into the cell environment by bursting of the cell membrane.

Question 3

Which tests can be used for investigation of the cause of haemolysis?

Answer 3

The osmotoc resistance test (fragility test)
Ham test (acid serum lysis)
the sucrose lysis test
mechanical resistance test

Question 4

What is physiological solution?

Answer 4

0,9”NaCl –> isotonic solution, samme hydrostatic pressure as blood.

Question 5

Hypotonic solution?

Answer 5

RBC absorb water through membrane to equalize the pressure. RBC volume increase and the cell burst.. Hemoglobin is released into the solution –> red with debris.

Question 6

Hypertonic solution?

Answer 6

intracellular water passes through the membrane into the solution. Volume decrease and cell shrinks –> star shape and breaks down.

Question 7

What is the most common disorder of the blood?

Answer 7

Anemia

Question 8

3 Main classes of anemia?

Answer 8

• exessive blood loss
• exessive blood cell destruction
• deficient red bloodcell production

Question 9

The cause of anemia is determined by?

Answer 9

patient history, clinical and physical examination, haematologic lab results.

Question 10

Which haematologic laboratory tests can be used?

Answer 10

concentration of haemoglobin, haematocrit, RBC, reticulocyte count, MCV, MHC, MCHC.