Organ explant cultures Flashcards
What is the connection between explant cultures and the 3R principle?
Alternative method that eliminate animal suffering during experiments, but allow investigation of intact organ, tissue and cell systems corresponding to the situation in Vivo.
Which systems does explant cultures refer to?
ex vivo systems
In which environment are explants cultured?
In a thermostat incubator at 37 C with a 5% CO2 atmosphere. Humid environment.
What is the result of DNA (gene constructs) electrophorated into tissues?
Misexpress genes, inhibit protein function, inhibit translation.
How can explants be manipulated?
beads soaked in signalling proteins, separation of epithelium and mesenchyme, heterothropic/ chronic recombination. Cels can be transplanted into specific regions of explants.
In which studies are explant cultures frequently used?
molecular basis of development
principle of tissue homeostasis
investigation of medical disorders at cell and molecular levels
What does the culture medium consist of?
DMEM (dulbecco’s modified eagle’s medium)
glutamine
pen/strep
10% fetal calf serum
Why does explant micromanipulation techniques have a great future?
easy culturing easy investigation reliable results support of the 3R principle GLP approaches
Where can explant cultures be used?
Stemcell-based tissue-engeneering –> creation of organs and tissues for transplantation
What is doxorubicin?
anthracycline antibiotics used in cancer therapy that blocks the cell cycle due to DNA damage.
What is the target of chemotherapies?
Cancer cells with high proliferation activity and rapid renewal.
Who were awarded by the Nobel Price for discoveries related to cell cycle and it’s regulations and when?
2001
Leland Hartwell, Tim Hunt, Paul Nurse
Give an example of another ex vivo approach in cellular and molecular physiology?
Organ slices –> allows testing of specific clusters of cells within an organ and enables easy observation of cell population.
