count of leukocytes Flashcards
What are leukocytes?
WBC –> mobile units of the body’s immune defence system
How do they function?
identify and defend against invasion of pathogens by phagocytizing or causing destruction of the foreign agents.
Explain term Cleanup crew?
They remove debris resulting from dead or injured cells and it is important for wound healing and tissue mainenance and repair.
What is used to count the cells?
Bürker chamber.
Which solution is used and what does it ensure?
Türk solution, stains the cells nuclei and dissolve the blood sample ( destroy RBC)
How is the bloodsmple treated?
by anticoagulant.
How is it done?
By counting the cells in 50 large squares using the Bürker rule and meandering movements.
Formula for WBC if you have 80 cells pr one square and 20 times dilluted blood ?
80/50 x 250 x 20 x 10^6 = 8x10^9
REMEMBER 10^9 !!! (Not like RBC 10^12)
How is WBC measured in automatoc hematological analyzer?
By identification according to optic characteristics of the cells. (morphology)
What does WBC depend on?
physiological/pathological conditions of the animal, gravidity, circadian alterations, nutrition, temperature, hypoxia, stress.
What does NOT inflict the WBC?
Not sex dependant!
Leukocytosis?
increased number of leukocytes
Leukopenia?
decreased number of leukocytes
Leukemia?
malign proliferation of blood cells (usually white)
Myeloid leukemia?
purposeless proliferation of one or more of the nonlymphoid marrow cell lines
