Cell Homeostasis

Question 1

What is homeostasis?

Answer 1

Dynamic constancy of internal environment in cells, tissues, organs and entire body.

Question 2

What effect has detergent on animal cells?

Answer 2

Degrades cell membrane and shrinks the cell.

Question 3

What effect has etanol on animal cells?

Answer 3

Cell shrinks. It is not a hypertonic solution but alcohol dehydrates the cell.

Question 4

1,5% NaCL

Answer 4

Hypertonic solution. Cell shrinks because water travel outside the cell because of the higher amount of solutes

Question 5

Distilled H2O?

Answer 5

Hypotonic solution –> Haemolysis

Question 6

What is physiological solution?

Answer 6

0,9% NaCL

Question 7

What must be considered in the case of blood sample collection and sample treatment?

Answer 7

To have a cell friendly and homeostatic environment.

Question 8

Name 5 different kinds of cell lysis based on the causing agent?

Answer 8

Osmotic
Physical (ultrasound, temperature)
chemical( reaction with membrane lipids)
toxic (bacterial, protozoa toxins), immunological(membrane defects)

Question 9

Name the four major buffer systems in mammalian bodies?

Answer 9

Bicarbonate buffer (carbonic acid) primary ECF buffer.
Protein buffer primary ICF, but also ECF.
Haemoglobin buffer primary buffer against carbonic acid changes
Phosphate buffer important urinary buffer, also ICF.

Question 10

What is PSB?

Answer 10

Phosphate buffered saline - an isotonic, non-toxic solution for ex vivo works with mammalian cells/tissue/organs. Maintain constant pH.