mid 2 review Flashcards
how does compliment kill
opsonization and anaphalatoxins
where are complient proteins
circulating and membrane bound
what are compliment proteins made by
liver hepatocytes
tissue macrophages
blood monocytes
epithelial cells
the two heads of c1q must bind to what
one igm - planar and staple form exposes CH2
or two close igGs (IgG3)
what does MBL have
two protesases
MASP 1 and 2 that activate when bound to terminal mannose residues
MBL and C1q
structurally homologous
steps of alternate pathway
C3b spontaneously produces
factor B binds
factor d cleaves to c3bBb
this normally dissocaites but stabilized by properdin (factor P)
now it cleaves more C3 (positive feedback loop)
what are the way so of regulating complement
C1 dissociation
DAF- decay accelerating factor of C3 convertases in lectin and classical
factor I cofactor- breaks up C3b and C4b
protectin- inhibitsMAC and poly C9
breaknig down anaphalatoxins by carboxypeptidase
what are the functions of compliment
removal of ag:ab complexes: RBCs bind complement
activate B cell signalling by activating compleemnt receptors
removal of necrotic and subsceullular memnrane
response to virus by all 3 types of compliment
at the _ level, HLA = MHC
protein
at the _ level HLA = H2
DNA
how many classes of MHC are ther =e
3
a and b chain of MHC I
a is HLA-A, HLA-B, HLA-C and B2 microglobulin chain that is invariant and coded by a non MHC gene
a and b chain of MHC II
a in HLA-DR is non polymorphic
a and b in HLA-DP and HLA-DQ is polymorphic
what occurs w the MHC receptor genes
no gene rearrangement
what MHC expressed on all cells
MHC I
how can MHC II be expressed on cells its not supposed to
it can deputize cells that arent pAPC by IFN Y
polygenic
many genes in the complex
polymorphic
many alleles that can make up the gene q
what MHC are anchor residues in
MHC I on the ends at 2 and 9
H bonding
less variability
shorter peptides
MHC II conserved residues
througout the peptide
what MHC is endogenous peptides
MHC I
process of MHC I antigen and receptor
peptide is into proteosome and TAP1/2 brings it into the ER
using ATP
process of MHC II antigen and recepto
a and b chain made in ER
assocaite w invariant li or CD74 that goes into the golgi and becomes CLIP and then peptide is endoctosed and associates w acidic protease vesicles that degrade it and HLA-DM catalyzes its switch w CLIP
what MHC leads to cytokine and inflamation
MHC II
what MHC leads to perforins and granzyme release
MHC I
what is cross presentation
dendrictic cells TRANSFER exogenous antigens on MHC I to activate Th cells
what MHC allele makes it more likely to deveop ankylosing spondylitis
HLA-B27
what MHC allele makes it more likely to not respond to hep B vaccine
HLA-DR3
what animal has limited polymorphism in their MHC genes
cheetas
tasmanian devils
transmit cancer through bites
have no MHC I activity
no NK cell activity
