extra 2 Flashcards
graft bw genetically identical
allograft
regular graft
first set reejction
second set
revascularization
healing
resolution
revasculariaxtion
cellular infiltration
thrombosis and necrosis
cellular infilatration
thrombosis and necrosis
what tests showed us that T cels mediate graft rejection
adoptive transfer experiment
MHC differences found in
heterozygous outbred populations
sensitization stage of graft rejection
CD4,8+ cells recognize ALLOANTIGEN
proliferate
5% recognize antigenic MHC (ALLORECOGNITION) directly or indirectly
form memory T cells
effector stage of graft rejection
mediated by massive infiltration of recipeitn cells
macrophages
neutrophils
ADCC
lytic enzymes
apoptosis
CD4,8+
compliment
hyperacture rejection
preexisting antibodies acivate compliment BEFORE REVASCULARIZATION
within 24 hrs
what mediates hyperactute rejection
antibodies
what mediates acute rejection
t cells
timeline for acute rejection
7-10 days
what mediates chronic rejection
humoral or cell mediated
acute rejection steps
massive infiltration of lymphoxttes and macrophages
-Th activation and effecotr stage steps
what are the viruses in pigs
PERVs
how do we remove enzymes that make the sugars in pig cells
homologous recombination
prevent the previously acquired immunity
how can we elimininate lymphocytes
total lymphoid irradiation
what is total lymphoid irradiation used for
bone marrow transplant and graft versu host disease
removes lymphocytes and allows donor stem cell to form its own new imune system
what are the generla immune suppresant drugs
azathioprine and cyclophosphamide
both w corticosteroids
azathiorprine
mitotic inhibitor stops b and t cell proliferation
cyclophosphamide
inserts into DNA helix and disrupts it
whats better, azathioprine w cortico or cyclophosphamide w cortico
cyclophosphamide
what are the two names of teh specific immunosupressive drugs
belatracept
OKT3
belatracept
soluble CTLA4 fusion protein- induces T cell anergy
OKT3
mAb for CD3- depletes T cells prior to transplant
