microscopy

Question 1

what does the term magnification mean?

Answer 1

the number of times which an image is bigger than the actual object

Question 2

what does resolution mean?

Answer 2

the ability to distinguish between 2 points close together

Question 3

what is the maximum resolution for electron and light microscope?

Answer 3

electron- 0.5nm

light- 200nm

Question 4

describe what the differences of resolution in electron and light microscope does

Answer 4

electron has 0.5nm meaning that points very close together can still be distinguished while in light points only up to 200 nm can be distinguished

Question 5

what type of specimen is used in light and electron microscope?

Answer 5

light- alive

electron-dead

Question 6

explain how wavelength affects ability to see structures

Answer 6

when a structure is larger than the wavelength, the structure is able to disrupt the wavelengths and can be seen
* the opposite is true

Question 7

what is the relative wavelength in light and electron microscope?

Answer 7

electron- specimen greater than wavelength
* can be seen
light- specimen smaller than wavelength
* cannot be seen

Question 8

what type of electron microscope are there

Answer 8

Scanning electron microscope- only shows surface

transmission electron microscope-shows internal features

Question 9

what is the mechanism of light microscope

Answer 9

light is shone upwards through lenses and the specimen and directly seen by the eye through the eye lens

• objective lens: 4x,10x,40x
• eye lens: always 10x

Question 10

what is the mechanism of electron microscope

Answer 10

electron beam shoot from the top, which passes through the specimen and onto the screen which creates a glow and later on converted into black and white

Question 11

why do we use a dead specimen when using electron microscope

Answer 11

1- water boils in vacuum causing dehydration of specimen if alive
2-other particles may interfere with the e-