Methods - Lecture 9/9/21

Question 1

Basophilic

Answer 1

Negatively charged tissue/structure (like nucleus) likes to bond with positive charged

Question 2

Acidophilic

Answer 2

Positively charged tissue/structure has an affinity to acidic dyes

Question 3

Hematoxylin

Answer 3

A basic dye, binds to neg structures

Question 4

Eosin

Answer 4

An acidic dye, affinity to positively charged stuff

Question 5

H and. E staining

Answer 5

Hematoxylin and eosin staining

Question 6

Substances that stain poorly in H and E

Answer 6

Lipids and mucous

Question 7

Lipid stains

Answer 7

Steatosis

Question 8

Periodic-acid Schaffer (PAS)

Answer 8

Stains molecules rich in carbohydrates (GAGs, proteoglycans and mucus)

Question 9

Silver stains

Answer 9

Used for reticular fibers and nerve cell processes (random nerve cells undergo rxn)

Question 10

Masson’s trichrome

Answer 10

Three colored stain that distinguishes between cell and cytoplasm
Cytoplasm/keratin- red
Nuclei- dark/purple
Connective tissue- blue

Question 11

Elastic fiber stain

Answer 11

Shows the elastin proteins in stretchy tissue

Question 12

Immunohistochemistry

Answer 12

Uses antibody for specific structures

Question 13

Examples of artifacts

Answer 13

• Cracks/tears
• Folds
• Sectioning defects
• Poor fixation