MCAT BIO CH. 5 PART 1 Flashcards
What is a virus?
An obligate intracellular parasite
A vrirus can reproduce outside a cell. T/F
They are obligated to reproduce within (intra) a cell
What must occur for a virus to begin reproducing?
Commandeer the cellular machinery of the host and make copies of themselves
What is the definition of a virus to remember?
A virus is an obligate intracellular parasite that relies on host machinery whenever possible
Some viruses can store what?
ATP in their capsid
How is the viruses genome protected?
Nucleic acid genome packaged in a protein shell
A virus is inside its host cell. T/F
A virus is not inside a host cell, it contains only its genome
What type of nucleic acid do mature viruses have?
Does not contain nucleic acid other than its genome
What is a limiting factor of viral genomes? What size are viruses?
Size; small
What is a difficulty with the exterior protein shell of virus?
A rigid structure of fixed size that cannot expand to accommodate a larger genome
What is the likely result if a visa genome is tripled in size?
Viral genome will probably no longer fit within the normal viral structures
What is a bacteriophage?
Virus that infects bacteria
To adapt to size constraint, how has viruses adapted to?
Viral genomes have evolved to be extremely economical
What are the two adaptation for the viral genomes?
- Few genes
2. Encoding
What is the few genes adaptation fo viral genomes?
Viral genome to carry very few genes and for the virus to rely on host-encoded proteins for transcription, translation and replication
What is the encoding adaptation fo viral genomes?
Ability to encode more than one protein in a given length of genome
How can viral genomes encode more than one protein in a given length of genome?
Utilizing more than one reading frame within a piece of DNA so that genes may overlap with each other
What is capsid?
Protein coat surrounding the viral nucleic acid genome
What is used to classify viruses?
External morphology of the capsid
How is the capsid made?
From repeating patterns of only a few protein building blocks
How do helical capsid look? How do polyhedral capsids look like?
Helical capsids are rod-shaped
Polyhedral capsids are multiple-sided geometric
Which virus is commonly used in research and what is it?
T4 bacteriophage and its host is bacterium E. coli
What do tail fibers do for a virus?
Attach to the surface of the host cell
What do base plate do for a virus?
Attach to the surface of the host cell
What do sheath do for a virus?
Contracts using the energy of stored ATP and inject the genome into the host
The viral capsid is composed of….? The viral genome is composed of…?
proteins; nucleic acids
What do many animal viruses possess?
Envelope that surrounds the capsid
What is the envelope on animal viruses?
Membrane n the exterior of the virus derived from the membrane of the host cell
What does the envelope on animal viruses contain?
Phospholipids, proteins, carbohydrates, proteins encoded by the viral genome
What is budding based on animal viruses?
The process of acquiring envelope on the virus through the host cell membrane
How do some viruses leave their de-envelopped capsid inside the host cell?
By fusing their envelope with the host’s plasma membrane
What are naked viruses?
Viruses which do not have enveloppes
Which ones are naked viruses?
All phages and plant viruses
Why are phages and plant viruses are naked viruses?
Because they infect hosts with cell walls; when virus leaves the cell, the cell wall is destroyed and membrane ruptured
What is the first step in viral infection?
A virus binds to a specific receptor on the cell surface
What happens the virus binds to the cell surface receipt?
Virus all be internalized by either fusion or receptor-mediated endocytosis
Why might the antibodies to a viral capsid be ineffective in blocking infection?
It suggests that the virus is enveloped, so the antibody cannot reach its epitope on the capsid surface in infectious virus
From where do our immune system recognize for viruses?
The viral surface is also important for recognition by our immune system
Why can only specific species or specific cell types are susceptible to infection?
Only cells with a receptor that matches the virus will become infected
How do the viral genome produce energy and replicate?
Viral genome contains genes that redirect the infected cell to produce viral products
What is the first step to the bacteriophage life cycles?
Attachment or absorption
What is attachment or absorption based on bacteriophage life cycles?
Binding to the exterior of a bacterial cell in a process
What is the second step to the bacteriophage life cycles?
Penetration or eclipse
What is penetration or eclipse based on bacteriophage life cycles?
Injection of the viral genome into the host cell
Why is the second step to the bacteriophage life cycles also called eclipse?
Because the capsid remains on the outer surface of the bacterium while the genome disappears into the cell
After the penetration step, what two options can the phage follow?
- Lytic cycle
2. Lysogenic cycle
What happens as soon as the phage genome entered the host cell?
Host polymerases and/or ribosomes begin to rapidly transcribes and translate it
What is hydrolase?
A hydrolytic enzyme that degrades the entire host genome
Hydrolase is an example of what…?
An early gene
What is an early gene?
Genes that are expressed immediately after infection which includes any special enzymes required to express viral genes
How are multiple copies of the phage genome produced? What else is produced at the same time?
Using the dNTPs resulting from degradation of the host genome; abundance of capsid proteins
What do the capsid proteins do after an abundance of capsid proteins are made?
Each new capsid automatically assembles itself around a new genome
What enzyme is produced once each new capsid automatically assembled itself around a new genome?
Lysozyme
What is lysozyme an example of?
Late gene
What is the lysozyme?
Destroys bacterial cell wall
What happens when the lysozyme breaks the bacterial cell wall?
The host bacterium bust because osmosis is not counteracted by the protection of the cell wall
The lytic cycle is an efficient way to do what?
For a virus to rapidly increase its numbers
What is the problem with the lytic cycle?
All host cells are destroyed
What is a prophage?
Phage genome incorporated into the bacterial genome during lysogenic cycle
What is the host called after the prophage has occurred?
Lysogen
How is the prophage after it has occurred infiltrated the host genome?
Silent
Why is the prophage dormant?
Transcription of phage genes is blocked by a phage-encoded repressor protein
What does the phage-encoded repressor protein bind to?
Binds to specific DNA element in phage promoters
Every time the host cell reproduces itself in the lysogenic cycle….?
The prophage is reproduced too
What happens when the prophage becomes activated?
It removes itself from the host genome and enters the lytic cycle
What is excision?
The prophage removes itself from the host genome
Why is a downside from the lysogenic cycle?
When the viral genome activates, excising itself from he host genome may cause it to take part of the host genome along with it
What is transduction?
Codes for a trait that the new infected host did not previously possess
What is important about the tissue-specificity of animal viruses?
Due to the distribution of receptors necessary or absorption
Animal viruses enter the cells by what?
Endocytosis
What is endocytosis?
The host cell engulfs the virus and internalizes it
What happens once the animal virus is inside the hose?
Viral genome is uncoated
What does it mean when the animal virus is uncoated?
Its released from the capsid
If the animal virus doesn’t go through endocytosis, how does it get into the cell?
Fuse with the plasma membrane to release virus into the cytoplasm
What are the methods the animal virus can infiltrate the genome when its released into the cytoplasm?
Lytic cycle, productive cycle or lysogenic cycle
What is the productive cycle?
Similar to the lytic cycle but does not destroy the host cell
Why is the productive cycle possible?
The enveloped viruses exist the host cell by budding through the host’s cell membrane and becomes coated with the membrane in the process
How does budding not destroy the cell?
The lipid bilayer membrane can reseal as the virus leaves
What is provirus?
Analogous to a prophage, the dormant form of the viral genome
What does the + RNA Viruses must do?
Must encode RNA-dependent RNA polymerase and do not have to carry it
What is the structural component of + RNA virus?
A single stranded RNA genome
What is the simplest type of viral genome?
+ RNA virus
What is an + RNA virus?
A piece of single-stranded viral RNA which serves as mRNA
The viral genome acts directly as what, in the animal cell? (For + RNA virus)?
As mRNA
What does it mean when the genome is infective?
Injecting an isolated genome into the host cell with result in virus production
In order for the virus to replicate itself, one of the proteins it encode must be….?
An RNA-dependent RNA polymerase
What is the role of an RNA-dependent RNA polymerase protein?
To copy the RNA genome for viral replication
If a viral genome is (+) strand RNA, what is used as a template by the RNA-dependent RNA polymerase?
To make (+) strand copies of the genome, the virus needs the complementary strand as a template: (-) strand RNA
If the viral genome needs (-) strand, how does the dependent RNA poly do it?
Produces a (-) strand intermediate before generating new (+) strand genomes
What does the (-) RNA Viruses must do?
Must carry RNA-dependent RNA poly and encode it too
The genome of a (-) RNA virus is _____ to the piece of RNA that encodes viral proteins?
Complementary
What is the template for the viral mRNA production in (-) RNA virus?
The genome of a (-) RNA virus is the template for viral mRNA production
When the - RNA viruses enter the host cell, what happens?
Create a + strand from the - genome
What must the retroviruses do?
Encode reverse transcriptase
What is an important example of retrovirus?
HIV, AIDS and HTLV
What are retrovirus?
+ RNA viruses that undergo lysogeny
How do retrovirus integrate into the genome?
Integrate as proviruses
What must the retrovirus have in order to integrate DNA genome?
Must also be composed of double-stranded DNA
The retroviruses enter the cell as RNA, how can they be composed of double stranded DNA?
Must undergo reverse transcriptase to make an RNA template
How is reverse transcriptase accomplished?
By RNA-dependent DNA polymerase ended by the viral genome
Why is retroviruses not required to carry his enzyme?
The viral RA genome can be translated by host ribosomes; reverse transcriptase may be made after the viral genome
What are the three main retroviral genes?
- gag
- pol
- env
What is the gag retroviral genes?
Codes for viral capsid proteins
What is the pol retroviral genes?
Polymerase codes for reverse transcriptase
What is the env retroviral genes?
Envelope codes for viral envelope proteins