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Microbiology 2 > M2 Lab Methods > Flashcards

M2 Lab Methods Flashcards

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Biology 101 flashcards
1
Q

what is procedure

A

handling specimen
lab processing and reporting
interpretation and use of info

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2
Q

what do u do in lab analysis

A

isolate and identify organism

  • sample
  • timing
  • handling
  • request form
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3
Q

how is a sample taken fro purulent infection (pus forming)

A

aspirate of pus - drain
prevent contamination
maintain anaerobes

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4
Q

how to sample mucosal infections

A

yeast, swab and oral wash

herpes, lesions swab

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5
Q

how to sample caries and per disease

A

complex pic involving mix of organisms

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6
Q

how to do samples for culture

A

results = 2-5 days
streaked blood agar (aerobic/anaerobic)
colonies then indie cosines
antibiotic sensitivity

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7
Q

what does culturing do

A

determine viability (aerobes Vs anaerobes)
component species
provides pure material (single colony)

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8
Q

what is culturing sequence

A

primary inoculum
second streak
3rd streak
streak to single colonies

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9
Q

what is general media

A

transport media
- sloppy agar, incl charcoal prevents desiccation and abs toxic agents

rich excess of nutrients

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10
Q

what is enrichment media

A

addition of blood/serum/extracts will support growth of fastidious organisms
blood agar also - haemolytic profile determined

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11
Q

what si selective media

A

presence of specific substance permits the growth of one organism over another

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12
Q

what re some examples of selective media

A

mannitol salt agar

salmonela-shigella

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13
Q

what is differential media

A

incroportio of chems produces visible changes in colonies that facilitate identification

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14
Q

what are some examples of differentiate media

A

macconkay agar
- bile salts & crystal violet
eosin & methylene blue

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15
Q

what can be used for identification

A

microscopy - single colony or pus sample

  • pure culture
  • shape, size, grouping
  • structures
  • staining
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16
Q

what are the cocci structures

A

single
pair
cluster
chains

17
Q

what are the rod bacilli structure

A

single
chain
curved rod
spiral

18
Q

what is one species with many colony phenotypes

A

bacillus subtilis

19
Q

what is metabolic profiling

A

biochem characterisation

utilisation of carbon sources and amino acids

20
Q

what is exo enzyme production

A

catalase
coagulase
hydrolysis of lipid
urease

21
Q

whats urease

A

urea converted to ammonia & CO2

22
Q

what causes fermentation

A

lactose

23
Q

what are serological tests

A

host immune response to Ag by rising of Abs

24
Q

what are Ab specific to

A

microbe or single component

25
Q

what else does serological tests do

A

detects presence of sp IgM Ab to virus
demo in vitro bu agglutination
rapid detection of viruses
serotype bac indeitifacton

26
Q

what are immunological techniques

A 
difficult to identify pathogens 
artificially raised sp antibodies 
- agglutination reactions
- DFA test
- ELISA
27
Q

what is aggltination

A

antibody to antigen with latex bead - red blood cell

caring clot

28
Q

what is DFA fluorescence tect

A

one step - org expo to Ab with attached dye, organism bind toAb and be seen

two step - similar to one step Ab at start not dye, instead secondary Ab dyed

29
Q

what is ELISA

A

enzyme linked immunosorbent assay

30
Q

how do u do ELISA

A 
coat wells with antigen
wash
add antibody test 
wash
add enzyme conjugate 
wash
add chromogenic enzyme substrate and read colour change
31
Q

what are virulence test

A

virulence factor can be critical in pathogenesis (adhesion, toxin)
in vitro antibody recognition, DNA hybridisation

32
Q

what is DNA technology

A

sp primers amplify sp peice dna

PCR

33
Q

what is MALDI-TOF

A

matrix assisted laser desorption ionisation time of fight

34
Q

what are the features of MALDI_TOF

A

powerful
rapid
precise
cost effective

35
Q

what does madli tof do

A

gen series of ions from a sample dept on its constituents
sep ions accord to mass
detects proteins
results in characteristic signature

Microbiology 2 (23 decks)

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