M&R 1.2 - Membrane Proteins, Membrane Asymmetry and the Cytoskeleton Flashcards

1
Q

What are the functions of membrane proteins?

A
  • Facilitated diffusion
  • Maintain ion gradients
  • Specificity (responses)
    (Functional evidence)
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2
Q

What are the biochemical evidences for membrane proteins?

A
  • Membrane fractionation and gel electrophoresis

- Freeze Fracture

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3
Q

How can proteins be mobile in bilayers? (4)

A
  • Conformational changes
  • Rotation
  • Lateral Diffusion
  • NO FLIP FLOP DUE TO LARGE HYDROPHILIC MOIETIES
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4
Q

How is the mobility of proteins restricted in the membrane? (4)

A
  • Aggregates of proteins form together which decreases movement
  • Tethering
  • Interactions with other cells (stick together)
  • Lipid mediated effects
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5
Q

What is tethering?

A

Membrane proteins hold on to the basement membrane

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6
Q

What is meant by lipid mediated effects?

A

Membrane proteins separate into cholesterol poor regions/ fluid phase

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7
Q

Where can membrane proteins be located in the membrane?

A
  • Peripheral

- Integral

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8
Q

Describe peripheral membrane proteins (3)

A
  • Surface bound by ionic or hydrogen bonds
  • Associated, not integral
  • Removed from bilayer by a change in pH or ionic strength
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9
Q

Describe integral membrane proteins (2)

A
  • Interact with hydrophobic areas

- Have to be removed by aggressive means e.g. Detergents/ organic solvents as they compete for non-polar interactions.

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10
Q

How are membrane proteins inserted into the bilayer?

A
  • Secretory pathway
  • Stop transfer signal reached
  • Protein is translated in cytoplasm and leaves stop transfer signal in endoplasmic reticulum therefore spanning the membrane
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11
Q

Describe the stop transfer signal

A

18-22 highly hydrophobic amino acids

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12
Q

Describe the amino acids that make up transmembrane proteins (4)

A

SHUP

  • Small
  • Hydrophobic
  • Uncharged
  • Polar
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13
Q

What happens if there are no signal peptidase sites?

A
  • No cleavage
  • N-terminal remains outside
  • C-terminal remains inside
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14
Q

What is topology?

A
  • N-terminal = in cytoplasm

- C-terminal = outside cell

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15
Q

What is the cytoskeleton?

A

A cage structure grafted on to the inside of the plasma membrane

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16
Q

Which proteins make up the cytoskeleton in an erythrocyte? (3 parts)

A
  • Actin and Spectrin
  • Attached to basement membrane by Ankyrin and Glycophorin
  • Bound to Band 3 and Band 4.1 respectively
17
Q

What are Ankyrin and Glycophorin?

A

Adaptor proteins

18
Q

What are the two types of haemolytic anaemias?

A
  • Hereditary Spherocytosis

- Hereditary Elliptocytosis

19
Q

What causes haemolytic anaemias?

A

A mutation in the gene for Spectrin

20
Q

Describe Hereditary Spherocytosis (4)

A
  • Depletes Spectrin coverage by 40-50%
  • Red Blood Cells become round
  • Increases cell lysis
  • Decreases cell lifespan
21
Q

Describe Hereditary Elliptocytosis (3)

A
  • Defect in the Spectrin molecule
  • Can’t form stable heterotetramers
  • Fragile cells