Lecture Anatomy 2 Flashcards
Robert Hooke
- The father of microscopy
- Coined term “cell” to describe the basic unit of life
Mattias schleiden and Theodore schwann
- All living things are composed of one or more cells
Hierarchy of life
- Tracing life down to the chemical level: the hierarchy of biological organization
- Ecosystem -> community -> population -> organism -> organ system -> organ -> tissue -> cell -> molecule -> atom
- Morphology of cells
o Different Sizes and shapes of cells
o Different function
o Structure determines function
- Cells differ in
o Ability to move
o Internal organization (prokaryotic versus eukaryotic (function)
o Metabolic activities (chemical reaction)
- Cells share
o Certain structural features and carry out many complicated process in basically the same way
Are the following organic molecules alive?
- Proteins, carbohydrates, lipids, and nucleic acids
o No
Cell theory
- All organisms are composed of cells and cell products
- All cells come from Previously existing cells
o Parent cell to two daughter cells (identical)
All organisms are composed of?
- Cells and cell products
Are all of the cells within an organism the same?
- No
How many distinct cell types make up the human body?
- 210
Life begins with?
- Cells
- Each cell performs all the functions necessary to sustain life
We all developed from?
- A single-celled human zygote formed by fertilization
o Smaller than a period found in your text books
- Zygote
o Eukaryotic cell formed by a fertilization event between two gametes
- Zygote develops into?
o A full blown organism with 100 trillion cells organized into complex tissues and organs
Two major categories of cells
- Prokaryotic cells and eukaryotic cells
- Difference between prokaryotic and eukaryotic cells
o Prokaryotic has no nucleus and eukaryotic has
o Prokaryotic cells:
only domains: bacteria and archea
Lacking nucleus
Simple cells
o Eukaryotic cells:
plant and animal kingdoms, including the fungi (multicellular molds and unicellular yeast
Have nucleus
What type of cells do humans have?
- Eukaryotic multicellular cells
Different cell size?
- Different function
- Complexity of what they do
Prokaryotic cells
- Consist of a single enclosed compartment that is surrounded by a plasma membrane
- Lacks a defined nucleus
- Dna is located where in prokaryotic cells?
o Nucleoid region
- What is the most numerous prokaryotes?
o Bacteria
- Bacteria
o Don’t have membrane bound compartments
o Have many proteins that are precisely localized in their aqueous interior or cytosol
- Cytosol
o The fluid in which organelles of the cell resides
Eukaryotic cells
- Contain a defined membrane-bound nucleus that is absent in prokaryotes
- Nucleus segregates the cellular DNA for the rest of the cell
Eukaryotic organisms Can be unicellular or multicellular
- Nucleus
o Command center of cell
o Contains genetic materials (RNA and DNA)
Three main parts of cells
- Plasma membrane
- The cytoplasm
- The nucleus
Plasma membrane
- Defines the extent of cell
o Inside – intracellular
o Outside – extracellular
o Interstitial fluid between cells - Separates the living cell from its nonliving surrounding
- Thing/flexible layer that separates the intracellular and extracellular compartments
What forms the plasma membrane?
- Molecule called phospholipids
- Phospholipid bilayers
- Hydrophilic head (outside cell)
- Hydrophobic tail (inside cell)
Phospholipids
- 2 fatty acid chains (tail = non polar or hydrophobic)
- A phosphate group (head = polar or hydrophilic)
Membranes consists of?
- Proteins embedded in the phospholipid bilayer
- Proteins make up 50% of membrane mass
- A fluid mosaic of lipids and proteins
Fluid mosaic
- Membrane phospholipids and proteins can drift about in the plane of the membrane
“fluid”
- Molecules can move freely within the membrane
“mosaic”
- A diversity of proteins exists within the membrane
Proteins found in plasma membrane
- Integral membrane proteins – firmly embedded
- Peripheral membrane proteins – loosely associated/not embedded
Integral membrane proteins
- Transmembrane
- Span entire width of membrane and contain both hydrophilic and hydrophobic regions
Peripheral membrane proteins
- Don’t span entire membrane
- Loosely associated with other proteins or lipid molecules
Functions of membrane
- Anchors the cells to form a common function
- Selective permeability
o Allow some substances to cross more easily than others (size or charge)
o Block passage of some substances altogether - Provides barrier against substances outside the cell
- Some act as receptor for cellular communication
- Transport
- Intercellular joining
Diffusion
- Movement of molecules from higher concentration to lower concentrating across membrane
- Tend to spread into available space
- Passive transport; no energy is needed
Facilitated diffusion
- Transport of some substances by specific transport proteins that act as selective corridors
- Traffic of some substances can only occur through transport proteins
- Glucose- require transport protein
- Passive transport
Osmosis
- (diffusion) Passive transport of water across a selectively permeable membrane from higher concentration to lower concentration
Survival of the cell depends on?
- Ability to balance water uptake and loss
Solution
- Solute and solvent
- Solute – gets dissolved
- Solvent – water does the dissolving
Isotonic solution
- Equal concentration of ions in solution and cell
Hypertonic solution
- Higher concentration of ions in solution than in cell
Hypotonic solution
- Lower concentration of ions in solution than in cell
Osmoregulation
- Control of water balance animals
Endocytosis
- Mechanism by which particles enter cells
3 forms of endocytosis
- Phagocytosis
- Pinocytosis
- Receptor-mediated endocytosis
Phagocytosis
- “cellular eating”
- Cell engulfs a particle and packages it within a food vacuole
- Most wbc
Pinocytosis
- “cellular drinking”
- Primarily used for absorption of extracellular fluids (ECF)
- Contrast to phagocytosis
o Generates very small vesicles
o Unspecific in the substances that it transports
- Receptor-mediated endocytosis
o Triggered by the binding of external molecules to membrane proteins
o Upon membrane proteins binding to certain molecules – membrane forms coted pit then pinch off to become coated vesicle (Low density lipoproteins or viruses
Exocytosis
- Mechanism that move substances (enclosed in a vesicle) out of the cell
- Vesicle migrates to plasma membrane
- Proteins from vesicle bind with membrane proteins
- Lipid layers from both membrane fuse
- Vesicle releases contents to outside of cells
Cytoplasm
- Cell-forming material
- Part of cell that lies internal to the plasma membrane and external to the nucleus
3 major elements that make up the cytoplasm
- Cytosol
- Organelles
- Inclusions
Cytosol
- Jelly-like, fluid-containing substance within the cell
- Consists of water, ions, and enzymes
- Makes up half of the volume of the cytoplasm
- Fluid In which other cytoplasmic elements are suspended
Cytoplasm contains nine types of organelles
- Mitochondria
- Ribosomes
- Lysosomes
- Cytoskeleton
- Centrioles
- Golgi apparatus
- Rough and smooth er
- Peroxisomes
Ribosomes
- Constructed of proteins and ribosome RNA
- Site of protein synthesis
- Composed of two subunits (60s and 40s) that fit together to form a functional ribosome
Type of ribosomes found in the cell:
- Free ribosomes
- Attached ribosomes
Free ribosomes
- Float in cytosol
- Make soluble proteins (function in cytosol)
Attached ribosomes
- Attached to rough ER and make membrane proteins or exported proteins
Ribosomes build all the cell’s proteins though a process
called?
- ???
- Translation
Antibiotics target the process of the?
- Ribosomes
Endoplasmic reticulum (ER)
- Network within the cytoplasm
- Extensive system of membrane-walled envelopes and tubes
Two distinct type of ER
- Rough ER
- Smooth ER
Rough ER
- Ribosomes stud the external surfaces
- Envelope stacks called cisternae
- Makes all membrane proteins and membrane
Smooth ER
- Consists of tubules in a branching network
- No ribosomes are attached
- No protein synthesis
- Store Ca(2+)
- Makes enzymes for lipid metabolism
- Including steroids
After the rough ER synthesizes a molecule it?
- Packages the molecule into transport vesicles
Golgi apparatus
- Packaging and shipping center
- Stack of 3 – 10 disk shaped membrane bound envelopes (cisternae)
- Works in partnership with ER; sorts products of rough ER at the cis end and sends them to proper destination from the trans end
Mitochondria
- Power plant of the cell
- Generates most of the cell’s energy (AT) via cellular respiration
- Enclosed by a double membrane: the inner membrane folds in forming shelf-like cristae
- Contains own DNA (maternal DNA)
Lysosome
- spherical membranous bags containing digestive enzymes, “demolition crew” that break down macromolecules
secretory lysosome
- found in white blood cells, immune cells, and melanocytes
lysosome functions
- digest ingested bacteria, viruses, and toxins
- degrade nonfunctional organelles
- breakdown glycogen and release thyroid hormone
- breakdown non-useful tissue (webbing between fingers and toes during fetal development
- breakdown bone to release Ca(2+)
peroxisomes
- ubiquitous organelles in eukaryotes that participate in the metabolism of fatty acids and metabolites
- membranous sacs containing oxidases and catalaes: “toxic waste plants”
peroxisome function
- detoxify harmful or toxic substances
- break down long chains of fatty acids (numerous in the liver and kidneys)
- neutralizes dangerous free radicals and break down poisons
free radicals
- highly reactive chemicals with unpaired electrons
cytoskeleton
- cell skeleton
- consisting of a network of rods/fibers that run throughout the cytosol
- provides mechanical support to the cell and maintain its shape and provides machinery for various cellular movements
cytoskeleton provides three types of protein rods (not covered by membranes)
- microtubules
- microfilaments
- intermediate filaments
microtubules
- cylindrical structures made of proteins called tubulin
- radiate from centrosome
- centrosome
o cell center
o organelles attach to and move along microtubules, which constantly assemble and dissemble
o thickest
microfilaments
- actin filaments
- filaments of contractile protein actin that interact with myosin to create cell division, perform endo- and exocytosis and play a role on pseudopod extension and retraction
- organelles also attach to and move along actin filaments which constantly assemble and dissemble
- thinnest
intermediate filaments
- protein fibers
- most stable and permanent
- help cells resist pulling forces
- provide tensile strength
- play a role in linking cells together
centrosomes
- spherical structure in the cytoplasm
- composed of centrosome matrix (outer cloud) and centrioles
- microtubules are anchored at the centrosome (microtubule organizing center)
centrioles
- paired cylindrical bodies forming a pinwheel array of nine triplets of microtubules = 27 short microtubules
- act in forming cilia and flagella
- organize mitotic spindle during mitosis
cilia
- motile appendage
- move in coordinated back and forth motion
- some extend from nonmoving cells
- human windpipe
flagella
- motile appendage
- propel the cell in a whiplike motion
inclusions
- temporary structure that are not present in all cell types
- may consist of pigments, crystals of proteins, and food store
- lipid droplets
o found in livers cells and fat cells and glycosomes
o glycosomes
store sugar in the form of glycogen = long chains of glucose
nucleus
- the central core or kernel
- control center of cell
- contains dna and directs the cells activities
- appr. 5um in diameter
nuclear envelope
- two parallel membranes separated by fluid filled space
nucleolus
- little nucleus
- in the center of nucleus
o conatins parts of several chromosomes
o site of ribosome subunit manufacture
nucleus contain chromatin
- dna in a cell Is packed into an elaborate, multilevel system of coiling and folding
DNA bonding
- purines: A and G
- Pyrimidines: C and T
- 3 hydrogen bonds between cytosine and guanine
- 2 hydrogen bonds between adenine and thymine
Chromatin composed of
- DNA and histone protein
Condensed chromatine contains
- Tightly coiled strands of DNA
Extended chromatin contains
- Uncoiled strans of DNA when DNA’s genetic code is copied onto mRNA
What is the highest level of organization of chromatic?
- Chromosomes
o Contain a long molecule of DNA
How many chromosomes are there in a typical human cell?
- 46 chromosomes
DNA controls the cell by?
- Transferring its coded information into RNA
The information in the RNA is used to?
- Make protein
The cell theory states…????
> > > >
What is reproduction?
- The birth of a new organisms
- Occurs much more often at the cellular level
FYI
- More cells in body than stars in milky way
- Past second, millions of your cells have divided into two
Before development and differentiation of cells you must have?
- Cell division
Cell division plays a role in?
- The replacement of lost or damaged cells
- Cell reproduction and growth
- Some multicellular organism can divide into pieces that then grow into new individuals
Before a parent cell divides…
- It duplicates all of its genetic materials
After cell division
- the two resulting “daughter” cells are genetically identical
what is as genome?
- Organisms complete set of DNA including all of its gene
Genome is located mainly on?
- Chromosome in the cells nucleus
Chromosomes are made of?
- Chromatin
- Combination of DNA and protein molecules
- Are not visible in a cell until cell division
Before a cell divides, it duplicate all of its chromosomes, resulting in two copies called?
- Sister chromatids
What happens to the sister chromatids when the cell divides?
- Separate from each other
Cell cycle
- Eukaryotic cells that divide undergoes an orderly sequence of events
Cell cycle consists of two distinct phases
- Interphase (90%)
- Mitotic phase (10%)
Interphase
- G1 – cell growth
- S phase – DNA synthesis; chromosome duplication
- G2
Mitosis
- The division of the chromosomes
- Preceded by interphase G1 S G2
Four distinct phases of mitosis
- Prophase
- Metaphase
- Anaphase
- Telophase
Prophase
- Asters are seen
- Chromatin condenses into chromosomes attached to one another by their centromere (sister chromatids + prtoeins = kinetochore
- Nucleoli disappears
- Centriole pairs separate and mitotic spindle is formed
- Nuclear envelope disappears
- Microtubules attach to kinetochores and begin moving sister chromatid to center of cells
Metaphase
- Sister chromatids cluster at the middle of the cell with their centromeres aligned at the exact center
- Arrangement of chromosomes along a plane midway between the poles is called the metaphase plate
Anaphase
- Centromeres of the sister chromatids split and each becomes a chromosome again
- Motor proteins in kinetochores pull chromosomes towards poles
Telophase
- Reversal of prophase
- Cleans up the after effects of mitosis
- Corresponding sister chromosomes attach at opposite ends of the cell
- New nuclear envelope forms around each of separated sister chromosomes
- Both sets of chromosomes unfold back into chromatin
Mitosis is complete after prophase-metaphase-anaphase-telophase, but cell division has yet one more step to complete. What?
- Cytokinesis
Cytokinesis
- Occurs during telophase
- The division of the cytoplasm
- Not part of mitosis
- Mitosis ends at telophase
Normal plant and animal cells have a cell cycle control system…
- Series of checkpoints
- When cell cycle control system malfunctions
o Cells may reproduce at the wrong time or place
o Benign tumor may form
What is cancer?
- A class of diseases in which a group of cells display the following characteristics o Uncontrolled growth o Invasion (intrusion on and destruction of adjacent tissues) o Metastasis (spread to other locatioin in the body via lymph or blood
Cancer is caused by?
- Breakdown in control of the cell cycle
- The cancerous cell ignores the cell checkpoints
Cancer cells?
- Divide excessively
- Spread from a malignant tumor
Metastasis
- Spreading of cancerous cells
Cancer treantment
- Radiation therapy disrupts cell division
- Chemotherapy involves drugs that disrupt cell division
Cancer research
- Cancer cells are often grown in culture for study
Cancer prevention and survival
- Changes in lifestyle
- Not smoking
- Avoiding exposur to the sun
- Eating a high-fiber, low-fat diet
- Visiting the doctor regularly
- Performing self-examination
Your body has how many different cell types?
- 210
Cellular diversity
- Specialized functions of cells relates to the shape of cells and the arrangement of organelles
Cells that conncet body parts or cover organs
- Fibroblast
- Erythrocyte (RBC)
- Epithelial cell
Fibroblast
- Makes and secretes protein component of fibers
Erythrocyte (RBC)
- Concave shape provides surface area for uptake of the respiratory gases
Epithelial cell
- Hexagonal shape allows maximum number of epithelial cells to pack together
Cells that move organs and body parts
- Skeletal and smooth muscle cells
o Elongated and filled with actiin and myosin
o Contract forcefully
Cells that store nutrients
- Fat cell (adipocyte_
o Shape is produced by large fat droplet in its cytoplasm
Cells that fight disease
- Macrophage
o Phagocyte that moves through tissue to reach infection sites
Cells that gather information
- Neuron
o Has long processes for receiving transmitting messages
Cells of reproduction
- Oocyte
- Sperm
Oocyte
- Female
- Largest cell in the body
- Contains many copies of organelles for distribution to daughter cells
Sperm
- Male
- Possess long tail for swimming to the egg for fertilization
Youth
- Begin as a feritilized egg
- Cells in embryo are exposed to chemical signals that channel cells into specific pathways of development
- Cell specialization leads to structural variation of cell types
Aging
- A complex process casued by a variety of factors
- Free radical theory
- Mitochondrial theory
- Genetic theory
o Telomeres
o Telomerase
Free radical theory
- Damage from byproducts of cellular metabolism
- Radicals build up and damage essential molecules of cells
Mitochondrial theory
- Decrease in production of energy by mitochondria weakens and ages our cells
Genetic theory
- Proposes that aging is programmed by genes
Telomeres
- End caps on chromosomes that limit the maximum number of time a cell can divide
Telomerase
- Prevents telomeres from degrading
chromatin
DNa + Histone (proteins)
nucleosome
- histone + dna combination without the linker DNA
linker DNA
between nucleosomes
heterochromatin
- condensed/packed chromatin
euchromatin
- extended/loose chromatin
DNA
double stranded helix
protein
amino acid + AA + AA
nucleotides
- form DNA strands
- Purine and Pyrimidine
purine
adenine A
Guanine G
pyrimidine
Thymine T
Cytosine C
(CUT - Pyramid)
nucleotide pairings
A-T (2 hydrogen bond)
G-C (3 hydrogen bond)
autosome
- 22 copies of non-sex chromosome
sex chromosome
X + Y
TRisomy 21
3 copies of chromosome 21
- downsyndrome
cellular division consists of
mitosis
cytokenisis
why cell division
to repair
cell growth
Gene
sequence of DNA that codes for functional protein or functional RNA
dna to protein process
DNA (transcription) -> RNA -> mRNA -> (Translation) Protein
centromere
middle center of sister chromatids
telomeres
end side of sister chromatids
homologous chromosomes
matching pairs
homologous pair from mom + dad identical?
not identical but similar
homologous chromosome of sister chromatids identical?
yes
ribosomes are composed of?
two subunits
- 40 small subunits
- 60 large subunits
is ribosome membrane bound?
no
what is the central dogma of molecular biology?
DNA -> RNA -> mRNA -> Protein
DNA to RNA
RNA to mRNA
mRNA to Protein
- transcription in nucleus
- post transcriptional modification in nucleus
- translation in cytoplasm
with in the attached ribosome, what happens to proteins being made
- exocytosed
- contained within a vesicle
- embedded in the plasma membrane
which organelles contains cisternae?
- ER
- Golgi apparatus
- mitochondria
oxidase and catalase
oxidase convert free radicals to hydrogen peroxide
catalase converts hydrogen peroxide to H2O +02