Lecture 21: Limb Development 1 Flashcards

1
Q

What is considered the actual primordium of the limb?

A

Limb disc

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2
Q

If the limb disc is removed, can a limb still form?

A

Yes, via the morphogenetic field around the disc

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3
Q

T/F: If the limb field is removed, no limb will form.

A

True

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4
Q

Removing half the disc, dividing the disc into two halves, & fusing two discs together results in…?

A

Normal limb development

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5
Q

What initiates future forelimb development?

A

Tbx5

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6
Q

What initiates future hindlimb development?

A

Tbx4 & Pitx-1

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7
Q

What are the initial initiators of limb development?

A

Tbx5 & Tbx4

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8
Q

Expression of which molecules forms AP axis?

A

Gli-3 (anterior)

Hand-2 (posterior)

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9
Q

The AP axis is fixed _____ in development & the DV axis is fixed _____ in development.

A

Early

Later

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10
Q

What happens if you transplant a limb disc to the opposite side?

A

Normal limb forms with the AP axis reversed

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11
Q

What happens if you rotate a limb disc 180 degrees?

A

Normal limb forms with AP axis reversed

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12
Q

What did Slack establish via his “flank tissue” experiment?

A

Flank tissue posterior to limb disc organizes posterior edge of the disc (posterior organizing region)

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13
Q

What substance provides the gradient for the posterior organizing region?

A

Shh, activated by retinoic acid

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14
Q

T/F: The ZPA is the posterior organizing region in birds.

A

True

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15
Q

What signaling molecule determines the position of the ZPA?

A

Hoxb8

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16
Q

What does Shh induce the expression of in the ZPA?

A

Gremlin

17
Q

How does Gremlin affect the posterior part of the limb bud?

A

Inhibits BMP-2 which inhibits FGF-4 in AER

Inhibits Gli-3

18
Q

What is expressed in the anterior part of the limb bud to make the anterior part different from the posterior part?

A

Gli-3

19
Q

What do the dorsal and ventral parts of the ectoderm of the AER express?

A

Dorsal: Radical fringe
Ventral: En-1

20
Q

T/F: Removal of the AER results in continued outgrowth of the limb bud.

A

False, results in cessation of outgrowth of limb bud

21
Q

_____ determines morphology of the limb & _____ determines whether the limb grows.

A

Mesoderm

AER

22
Q

What do cells in the distal limb bud express?

A

Msx-1

23
Q

What creates the pattern formation in the limb?

A

Hoxd gene expression

24
Q

Which Hox gene is expressed throughout the entire limb?

A

Hoxd-9

25
Q

What does the stylopod form?

A

Humerus or femur

26
Q

What does the proximal zeugopod form?

A

Radius/Ulna or Tibia/Fibula

27
Q

What does the distal zeugopod form?

A

Carpals or tarsals

28
Q

What does the proximal autopod form?

A

Metacarpals or metatarsals

29
Q

What does the distal autopod form?

A

Phalanges

30
Q

When is mesenchyme of the limb bud determined?

A

Before closure of the neural tube

31
Q

What is the structure of the developing limb determined by?

A

Mesodermal components

32
Q

T/F: Ectoderm tells the mesoderm what to form/make.

A

False, mesoderm tells the ectoderm what to form/make

33
Q

What maintains the AER in a healthy state and where is it produced?

A

Apical Ectodermal Maintenance Factor (AEMF)

Mesoderm

34
Q

AER maintains the mesodermal cells in a _____ and _____ state.

A

Labile

Mitotic

35
Q

T/F: Apoptosis is not a mechanism for morphogenesis.

A

False, it is a method for morphogenesis