Lecture 21: Limb Development 1 Flashcards
What is considered the actual primordium of the limb?
Limb disc
If the limb disc is removed, can a limb still form?
Yes, via the morphogenetic field around the disc
T/F: If the limb field is removed, no limb will form.
True
Removing half the disc, dividing the disc into two halves, & fusing two discs together results in…?
Normal limb development
What initiates future forelimb development?
Tbx5
What initiates future hindlimb development?
Tbx4 & Pitx-1
What are the initial initiators of limb development?
Tbx5 & Tbx4
Expression of which molecules forms AP axis?
Gli-3 (anterior)
Hand-2 (posterior)
The AP axis is fixed _____ in development & the DV axis is fixed _____ in development.
Early
Later
What happens if you transplant a limb disc to the opposite side?
Normal limb forms with the AP axis reversed
What happens if you rotate a limb disc 180 degrees?
Normal limb forms with AP axis reversed
What did Slack establish via his “flank tissue” experiment?
Flank tissue posterior to limb disc organizes posterior edge of the disc (posterior organizing region)
What substance provides the gradient for the posterior organizing region?
Shh, activated by retinoic acid
T/F: The ZPA is the posterior organizing region in birds.
True
What signaling molecule determines the position of the ZPA?
Hoxb8