LEC EXAM #3 worksheet Flashcards
Which of the following regarding the Ames test is true?
It is used to identify mutants with restored biosynthetic activity.
When the ribosome reaches a nonsense codon, which of the following occurs?
The polypeptide is released
The F plasmid is involved in which of the following processes?
Conjugation
The formation of pyrimidine dimers results from which of the following?
Exposure to ultraviolet radiation
Which of the following is an example of a frameshift mutation?
Deletion of one nucleotide
Which of the following refers to the mechanism of horizontal gene transfer naturally responsible for the spread of antibiotic resistance genes within a bacterial population?
Conjugation
An operon of genes encoding enzymes in a biosynthetic pathway is likely to be which of the following?
Constitutive
Which of the following conditions leads to maximal expression of the lac operon?
Lactose present, glucose absent
All of the following are processes used to introduce DNA molecules into bacterial cells except:
Transcription
The enzyme that uses RNA as a template to produce a DNA copy is called:
Reverse transcriptase
Which technique is used to separate protein fragments based on size?
Polyacrylamide gel electrophoresis
Which technique uses restriction enzyme digestion followed by agarose gel electrophoresis to generate a banding pattern for comparison to another sample processed in the same way?
RFLP
The insulin produced by recombinant DNA technology is:
Identical to human insulin produced in the pancreas.
Which of the following types of medical items requires sterilization?
Needles
Which of the following is suitable for use on tissues for microbial control to prevent infection?
Antiseptic