LEC EXAM #3 worksheet Flashcards

1
Q

Which of the following regarding the Ames test is true?

A

It is used to identify mutants with restored biosynthetic activity.

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2
Q

When the ribosome reaches a nonsense codon, which of the following occurs?

A

The polypeptide is released

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3
Q

The F plasmid is involved in which of the following processes?

A

Conjugation

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4
Q

The formation of pyrimidine dimers results from which of the following?

A

Exposure to ultraviolet radiation

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5
Q

Which of the following is an example of a frameshift mutation?

A

Deletion of one nucleotide

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6
Q

Which of the following refers to the mechanism of horizontal gene transfer naturally responsible for the spread of antibiotic resistance genes within a bacterial population?

A

Conjugation

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7
Q

An operon of genes encoding enzymes in a biosynthetic pathway is likely to be which of the following?

A

Constitutive

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8
Q

Which of the following conditions leads to maximal expression of the lac operon?

A

Lactose present, glucose absent

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9
Q

All of the following are processes used to introduce DNA molecules into bacterial cells except:

A

Transcription

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10
Q

The enzyme that uses RNA as a template to produce a DNA copy is called:

A

Reverse transcriptase

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11
Q

Which technique is used to separate protein fragments based on size?

A

Polyacrylamide gel electrophoresis

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12
Q

Which technique uses restriction enzyme digestion followed by agarose gel electrophoresis to generate a banding pattern for comparison to another sample processed in the same way?

A

RFLP

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13
Q

The insulin produced by recombinant DNA technology is:

A

Identical to human insulin produced in the pancreas.

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14
Q

Which of the following types of medical items requires sterilization?

A

Needles

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15
Q

Which of the following is suitable for use on tissues for microbial control to prevent infection?

A

Antiseptic

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16
Q

Which of the following best describes a microbial control protocol that inhibits the growth of molds and yeast?

A

Fungistatic

17
Q

The decimal reduction time refers to the amount of time it takes to which of the following?

A

Reduce a microbial population by 90%

18
Q

Which of the following terms is used to describe the time required to kill all of the microbes within a sample at a given temperature?

A

Thermal death time

19
Q

Which of the following microbial control methods does not actually kill microbes or inhibit their growth but instead removes them physically from samples?

A

Filtration

20
Q

Which of the following peroxygens is widely used as a household disinfectant, is inexpensive, and breaks down into water and oxygen gas?

A

Hydrogen peroxide

21
Q

Which type of test is used to determine whether disinfectant solutions actively used in a clinical setting are being used correctly?

A

In-use test

22
Q

Which of the following combinations would most likely contribute to the development of a superinfection?

A

Short-term use of narrow-spectrum antimicrobials

23
Q

Which of the following routes of administration would be appropriate and convenient for home administration of an antimicrobial to treat a systemic infection?

A

Oral

24
Q

Which clinical situation would be appropriate for treatment with a narrow-spectrum antimicrobial drug?

A

Treatment of strep throat caused by culture identified Streptococcus pyogenes

25
Q

Which of the following terms refers to the ability of an antimicrobial drug to harm the target microbe without harming the host?

A

Selective toxicity

26
Q

Which of the following is not a type of β-lactam antimicrobial?

A

Glycopeptides

27
Q

Which of the following does not bind to the 50S ribosomal subunit?

A

Tetracyclines

28
Q

Which of the following antimicrobials inhibits the activity of DNA gyrase?

A

Nalidixic acid

29
Q

Which of the following is not an appropriate target for antifungal drugs?

A

Cholesterol

30
Q

Which of the following drug classes specifically inhibits neuronal transmission in helminths?

A

Avermectins

31
Q

Which of the following is a nucleoside analog commonly used as a reverse transcriptase inhibitor in the treatment of HIV?

A

Adenine-arabinoside