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BIOL 270 > LAB EXAM #2 CONT. > Flashcards

LAB EXAM #2 CONT. Flashcards

1
Q

How to perform sodium thioglycollate medum test?

A

TSA plate:
1. Streak for isolation

Catalase:
1. Add a drop of hydrogen peroxide to TSA plate

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2
Q

How to perform blood agar plate test?

A
  1. Streak for isolation
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3
Q

How to perform methyl red test?

A
  1. Inoculate MR-VP broth with org
  2. Incubate at optimum temp for 5 days
  3. After incubation, add methyl red
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4
Q

How to perform VP test?

A
  1. Inoculate MR-VP broth with org
  2. Incubate at optimum temp for 5 days
  3. After incubation, add VP A and VP B
  4. Shake and let sit for 15 minutes
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5
Q

How to perform fermentation test?

A
  1. Inoculate 6 fermentation broths with org
  2. Incubate at optimum temp
  3. Recheck at 24 and 48 hours
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6
Q

How to perform O/F test?

A
  1. Inoculate 2 tubes of O-F glucose with org
  2. Overlay 1 tube with oil
  3. Incubate tubes at optimum temp
  4. Recheck at 24 and 48 hours
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7
Q

How to perform Simmon’s citrate test?

A
  1. Inoculate citrate slant with org

2. Incubate at optimum temp

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8
Q

How to perform nitrate test?

A
  1. Inoculate nitrate broth with org
  2. Incubate at optimum temp
  3. After incubation, add Reagent A and Reagent B
  4. If no color change, add Reagent C (zinc)
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9
Q

How to perform starch agar test?

A
  1. Inoculate starch plate with streak in the center of the plate
  2. Incubate at optimum temp for 5 days
  3. After incubation, flood with Gram’s iodine
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10
Q

How to perform skim milk agar test?

A
  1. Inoculate skim milk agar plate with streak in the center

2. Incubate at optimum temp for 5 days

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11
Q

How to perform spirit blue agar test?

A
  1. Inoculate spirit blue plate with streak in the center

2. Incubate at optimum temp

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12
Q

How to perform urea test?

A
  1. Inoculate urea broth with org

2. Incubate at optimum temp for 5 days

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13
Q

How to perform phenylalanine test?

A
  1. Inoculate the phenylalanine slant with org
  2. Incubate at optimum temp
  3. After incubation, add 10% ferric chloride to slant
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14
Q

How to perform DNase test?

A
  1. Inoculate DNase plate with org

2. Incubate at optimum temp

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15
Q

How to perform decarboxylase test?

A
  1. Inoculate all 3 decarboxylase broths
  2. Overlay with oil
  3. Incubate at optimum temp
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16
Q

How to perform gelatin test?

A
  1. Inoculate gelatin stab with org
  2. Incubate at optimum temp for 5 days
  3. After incubation, refrigerate for 15 minutes
17
Q

How to perform indole test?

A
  1. Inoculate SIM agar with org
  2. Incubate at optimum temp
  3. After inoculation, add Kovac’s reagent
18
Q

How to perform sulfur reduction test?

A

After inoculation of SIM agar tube, check tube for black precipitate before finishing the rest of the indole test

BIOL 270 (26 decks)

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