LAB - sectioning

Question 1

Previously processed tissue, is trimmed and cut into uniformly thin slices or sections

Answer 1

Sectioning

Question 2

sectioning is also called as

Answer 2

Microtomy

Question 3

Where the embedded tissue is held in position

Answer 3

Block holder

Question 4

Instrument that perform the actual cutting of the tissue sections

Answer 4

Knife carrier and knife

Question 5

Lines up with tissue block in proper position with the knife, adjust proper thickness

Answer 5

Pawl, ratchet feed wheel and adjustment screws

Question 6

• simplest microtome
• For small and large paraffin-embedded blocks
• Not for serial sections

Answer 6

Rocking

Question 7

• Most common microtome
• For paraffin embedded blocks

Answer 7

Rotary

Question 8

Most dangerous type of microtome

Answer 8

Sliding

Question 9

For celloidin embedded blocks

Answer 9

Standard sliding

Question 10

Hard and tough tissue blocks in all forms of media

Answer 10

Sliding

Question 11

For Small specimens embedded in osmium tetraoxide and embedded in plastic

Answer 11

Ultra thin

Question 12

• undehydrated tissues in frozen state
• Fats and heat sensitive tissue constituents

Answer 12

Freezing microtome

Question 13

Average temp of cryostat

Answer 13

20C

Question 14

Rotary microtome inside a cold chamber

Answer 14

Cryostat

Question 15

Maintained temp in a cold microtome/cryostat

Answer 15

-5 to -30C

Question 16

Sharp cutting edge capable of producing 2-4 um

Answer 16

Disposable blades

Question 17

Partially calcified tissue, paraffin and frozen sections for cutting 10 um and above

Answer 17

Safety razor blades

Question 18

Substance which can be smeared onto the slide so that sections stick well to the slides

Answer 18

Adhesion

Question 19

components of dried albumin

Answer 19

• dried albumin
• NaCl
• Thymol

Question 20

components of 1% gelatin

Answer 20

• gelatin
• glycerol
• phenol

Question 21

sections are dried and stored in 70% alcohol until it is ready for staining

Answer 21

dried albumin

Question 22

added to waterbath during flotation rather than applying it on slides

Answer 22

1% gelatin

Question 23

gelatin-formaldehyde mixture components

Answer 23

• gelatin
• formaldehyde

Question 24

slides are coated and allowed to dry at 37C for one hour or overnight prior to use

Answer 24

gelatin-formaldehyde mixture

Question 25

• aqueous detergent diluted to 0.01% concentration
• widely used in immunihistochemistry

Answer 25

poly-L-lysine

Question 26

APES

Answer 26

3-aminoprophylthriethoxysilane

Question 27

very useful in cytology, particularly for cytospin preparations of proteinaceous or bloody material

Answer 27

APES

Question 28

temp for floating on water bath

Answer 28

45-50C 0r 6-10C lower than the MP of wax

Question 29

temp of water bath for fishing out

Answer 29

10C lower than the melting point of wax

Question 30

correct positioning of the tissue section/ribbon on the slide

Answer 30

orientation

Question 31

deparaffinization and drying out of sections for delicate tissues

Answer 31

37C for atleast 24 hours

Question 32

temp for blower-type electric slide dryer

Answer 32

50-55C for 20-30 mins

Question 33

reagent used for post-mordanting

Answer 33

2.5-3% K2CrO4 for 24 hrs

Question 34

used primarily as mordant and secondary as fixative

Answer 34

post-mordanting

Question 35

in post mordanting what reagents are utilized when soaking for 5-10 mins

Answer 35

• aq. solution of HgCl2 (mercuric chloride)
• aq. picric acid