DEHYDRATION Flashcards
must have high affinity with water and must have increasing strengths
dehydration
volume fo dehydrating tissue
10x
alcohol that is clear, colorless, flammable fluid
ethanol
considered to be the best dehydrating agent because it is fast-acting, it mixes with water and many organic solvents, and it penetrates tissues daily.
ethanol
alcohol that is fast acting and not poisonous
ethanol
what is added to ethanol to make it a tissue softener
4% phenol
toxic, for smear prep, for blood and tissue films
methanol
best for all around ethanol substitute and is used in microwave processing schedules
isopropyl alcohol
dehydrating agent for plants and animals
butyl alcohol
sltn for complete dehydration with alcohol found 1/4 at the bottom of the container
- anhydrous copper sulfate crystals covered with filter paper
test for complete dehydration with alcohol for full saturation of alcohol with water
blue discoloration of crystals
prolonged storage at lower conc. may macerate tissue
true
EGME
ethylene glycol monoethyl ether
other name for EGME
cellosolve
dehydrating agent that is rapid but toxic
cellosolve
dehydrating agent that iscombustible at 110-120F an allows longer storage
cellosolve
can affect reproductive system, fetal developmentmay be affected
cellosolve
more safer than EGME
propylene
time sched for dehydration
- graupner’s
- weisberger’s
miscible with H2O, alcohol, paraffin wax, xylol
Dioxane
miscible with alcohol, xylene, benzene, ether chloroform and acetone
THF
does not dissolve aniline dye and have offensive odor that may cause conjuctivitis
THF
soluble in water, alcohol, xylene, ether, benzene, cholorform, acetone
triethyl PO4
