FROZEN SECTION / CELL BLOCKING

Question 1

Advantage of frozen section

Answer 1

• Rapid Production of Intraoperative Diagnosis
• Diagnostic and Research Histochemistry
• Immunofluorescence and Immunohistochemistry
• Specialized Silver Stains in Neuropathology

Question 2

disadvantage fo frozen section

Answer 2

• No Serial Sections
• Structural Details Tend to be Distorted
• Staining is rarely Satisfactory
• Freezing Artifact

Question 3

requires thickness of tissue for cold knife procedure

Answer 3

3-5 mm

Question 4

freezing agent for cold knife procedure

Answer 4

CO2

Question 5

temp of knife in cold knife procedure

Answer 5

-40 to -60C

Question 6

temp of tissue in cold knife procedure

Answer 6

-5 to -10C

Question 7

temp of environment for cold knife procedure

Answer 7

0 to -10C

Question 8

thickness of sections produced in cold knife procedure

Answer 8

10u (dew line)

Question 9

Operating Temperature:

Answer 9

10 to -30C

Question 10

Optimum Working Temperature

Answer 10

-18 to -20C

Question 11

temp for fatty tissue, skin with fatty subcutis, fatty breast

Answer 11

-35C

Question 12

temp for brain, lymph nodes, liver, spleen, kidney, testis, uterine cutterings, soft cellular tumor, and thyroid

Answer 12

-5 to -15

Question 13

temp for muscle, connective, pancreas, uterus, cervix, non-fatty skin, non-fatty breast tissue, ovary, prostate, tongue, and gut

Answer 13

-15 to -25

Question 14

involves the preparation of cells used for diagnosis when cancer is suspected or to provide evidence of metastatic disease

Answer 14

cell block

Question 15

performed in conjunction with Pap’s stain

Answer 15

cell block

Question 16

Various cell blocking methods

Answer 16

• gel based methods
• coagulation based methods
• preformed supporting media
• for already processed cytology preparation

Question 17

Types of fresh, unfixed cytology specimens with reference to cell blocking

Answer 17

• fresh unfixed tissue specimen with singly scattered cells / fragments
• fresh unfixed specimen with clot
• formalin fixed or fresh specimens with fatty fragments

Question 18

Most important and widely used staining in cytopath

Answer 18

Papanicolaou smear

Question 19

Spread of cancer cells from their original site, typically a tumor to other parts of the body

Answer 19

Metastatic disease

Question 20

Specimens for cell-block

Answer 20

• fnac/fnab
• sputum
• urine
• effusion fluid
• lavage and washings

Question 21

-performed in organs s/a thyroid, breasts, lymph nodes
- distinguish between benign and malignant cells

Answer 21

Fnab

Question 22

From respiratory samples and for diagnosis of lung cancer

Answer 22

Sputum

Question 23

Specimen for diagnosing urinary tract disorder, bladder cancer, and renal disease

Answer 23

Urine

Question 24

Utilized to identify the etiology of cancer metastasis or inflammatory conditions

Answer 24

Effusion fluids

Question 25

Procedures that involve flushing a specific body cavity to acquire specimen

Answer 25

Lavage/flushing