FROZEN SECTION / CELL BLOCKING Flashcards
Advantage of frozen section
- Rapid Production of Intraoperative Diagnosis
- Diagnostic and Research Histochemistry
- Immunofluorescence and Immunohistochemistry
- Specialized Silver Stains in Neuropathology
disadvantage fo frozen section
- No Serial Sections
- Structural Details Tend to be Distorted
- Staining is rarely Satisfactory
- Freezing Artifact
requires thickness of tissue for cold knife procedure
3-5 mm
freezing agent for cold knife procedure
CO2
temp of knife in cold knife procedure
-40 to -60C
temp of tissue in cold knife procedure
-5 to -10C
temp of environment for cold knife procedure
0 to -10C
thickness of sections produced in cold knife procedure
10u (dew line)
Operating Temperature:
10 to -30C
Optimum Working Temperature
-18 to -20C
temp for fatty tissue, skin with fatty subcutis, fatty breast
-35C
temp for brain, lymph nodes, liver, spleen, kidney, testis, uterine cutterings, soft cellular tumor, and thyroid
-5 to -15
temp for muscle, connective, pancreas, uterus, cervix, non-fatty skin, non-fatty breast tissue, ovary, prostate, tongue, and gut
-15 to -25
involves the preparation of cells used for diagnosis when cancer is suspected or to provide evidence of metastatic disease
cell block
performed in conjunction with Pap’s stain
cell block
Various cell blocking methods
- gel based methods
- coagulation based methods
- preformed supporting media
- for already processed cytology preparation
Types of fresh, unfixed cytology specimens with reference to cell blocking
- fresh unfixed tissue specimen with singly scattered cells / fragments
- fresh unfixed specimen with clot
- formalin fixed or fresh specimens with fatty fragments
Most important and widely used staining in cytopath
Papanicolaou smear
Spread of cancer cells from their original site, typically a tumor to other parts of the body
Metastatic disease
Specimens for cell-block
- fnac/fnab
- sputum
- urine
- effusion fluid
- lavage and washings
-performed in organs s/a thyroid, breasts, lymph nodes
- distinguish between benign and malignant cells
Fnab
From respiratory samples and for diagnosis of lung cancer
Sputum
Specimen for diagnosing urinary tract disorder, bladder cancer, and renal disease
Urine
Utilized to identify the etiology of cancer metastasis or inflammatory conditions
Effusion fluids
Procedures that involve flushing a specific body cavity to acquire specimen
Lavage/flushing
