LAB - FIXATIVES

Question 1

gas produced by the oxidation of methyl alcohol

Answer 1

formaldehyde

Question 2

___ in tissues lead to acidity which favors the formation of formalin heme pigments

Answer 2

hypoxia

Question 3

black, polarizable deposits that is formed when hypoxia in tissues lead to acidity

Answer 3

formlain heme pigments

Question 4

pure stock

Answer 4

40% formalin

Question 5

1:10 dilution

Answer 5

10% solution

Question 6

1:20 dilution

Answer 6

5% solution

Question 7

concentration for fixation of formaldehyde

Answer 7

10% formalin

Question 8

central nervous tissues and general post-mortem tissues for histochemical examination

Answer 8

10% formol-saline

Question 9

ideal with most stains including silver impregnation

Answer 9

10% formol saline

Question 10

duration of fixation of formol-saline

Answer 10

more than 24 hours (slow fixative)

Question 11

preservation and storage of surgical, post-mortem, and research specimens

Answer 11

10% neutral buffered formalin

Question 12

white precipitate due to prolonged storage

Answer 12

paraformaldehyde

Question 13

how to remedy paraformaldehyde

Answer 13

removed by filtration or addition of 10% methanol

Question 14

best fixative for frozen sections

Answer 14

10% neutral buffered formalin

Question 15

best fixative for iron pigments and elastic fibers

Answer 15

10% neutral buffered formalin

Question 16

routine post mortem tissues

Answer 16

formol-corrosive

Question 17

for lipids especially for neutral fats and phospholipids

Answer 17

formol-corrosive

Question 18

no need for washing out

Answer 18

formol-corrosive

Question 19

used to fix sputum

Answer 19

alcoholic formalin

Question 20

made up of two formaldehyde residues linked by three carbon chains

Answer 20

glutaraldehyde

Question 21

used in conjunction with osmium tetroxide

Answer 21

glutaraldehyde

Question 22

fixation time of glutaraldehyde

Answer 22

1/2 hour to 2 hours

Question 23

Small tissue fragments and needle biopsy when using glutaraldehyde

Answer 23

2.5% sltn

Question 24

Percent of. Concentration for Large tissues less than 4mm thick when using glutaraldehyde

Answer 24

4% solution

Question 25

percentage of concentration for normal electron microscopy in glutaraldehyde

Answer 25

3%

Question 26

percentage of concentration for immunoelectron microscopy when using glutaraldehyde

Answer 26

0.25%

Question 27

removal of mercuric deposits in tissue

Answer 27

De-Zenkerization

Question 28

most common metallic fixative

Answer 28

mercuric chloride

Question 29

for tissue photography and renal biopsy

Answer 29

mercuric chloride