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HSTCYT1 > H AND E STAINING > Flashcards

H AND E STAINING Flashcards

1
Q

major steps in routine H and E

A
  1. De-paraffinization
  2. Hydration
  3. Nuclear staining
  4. Differentiation/ decolorization
  5. Bluing
  6. Counterstaining
  7. Dehydration
  8. Clearing
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2
Q

reagent commonly used for bluing

A

ammonia water

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3
Q

composition of ammonia water

A
  • H2O
  • ammonium hydroxide
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4
Q

color of cartilage

A

Pink or light blue to dark blue

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5
Q

color of plasma cell and osteoblast

A

purplish pink

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6
Q

color of RBC, eosinophilic granules, keratin

A

bright orange red

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7
Q

color of cytoplasm

A

pink

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8
Q

color of nuclei

A

blue or blue-black

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9
Q

color of karyosome

A

dark blue

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10
Q

color of calcified bones

A

purplish blue

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11
Q

color of muscle fibers for H and E

A

deep pink

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12
Q

types of hematoxylin stain

A

A. aluminum hematoxylin solution
B. iron hematoxylin

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13
Q

recommended for progressive staining of tissue

A

aluminum hematoxylin solution

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14
Q

produces good nuclear stain

A

aluminum hematoxylin solution

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15
Q

mordant of ammonium hematoxylin solution

A

potash aluminum

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16
Q

for tissues subjected to acid decalcification

A

Erlich’s hematoxylin

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17
Q

for tissues that have been become acidic during prolonged storage in formalin

A

Erlich’s hematoxylin

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18
Q

ripening agent of Erlich’s hematoxylin

A

sodium iodate

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19
Q

stabilizer of Erlich’s hematoxylin

A

glycerine

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20
Q

2 solutions of Erlich’s hematoxylin

A
  • hematoxylin in absolute alcohol solution and heated
  • aluminum potassium sulfate in distilled water + glycerin and heated
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21
Q

used for routine nuclear staining, exfoliative cytology, and sex chromosomes

A

Harris’ Hematoxylin

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22
Q

staining time for Harris’ Hematoxylin

A

5-20 mins

23
Q

ripening agent for Harris’ Hematoxylin

A

Mercuric oxide

24
Q

color of Harris hematoxylin when ripened with mercuric chloride

A

dark purple

25
Q

stabilizer of Harris hematoxylin

A

4% glacial acetic acid

26
Q

used in sequence with celestine blue

A

Cole’s heamtoxylin

27
Q

ripening agent of Cole’s hematoxylin

A

Sodium perbonate

28
Q

prior to use, it should be filtered before use for long periods

A

Cole’s hematoxylin

29
Q

used to demonstrate the presence of cytoplasmic glycogen by special stain

A

Mayer’s hematoxylin

30
Q

regressive and progressive staining

A

Meyer’s Hematoxylin

31
Q

used for the demonstration of cytoplasmic glycogen

A

Mayer’s hematoxylin

32
Q

ripening agent for Mayer’s hematoxylin

A

sodium iodate

33
Q

used mainly for differential or regressive staining

A

iron hematoxylin

34
Q

uses acid alcohol as differentiator

A

iron hematoxylin

35
Q

types of iron hematoxylin

A
  1. weigert’s hematoxylin
  2. Heidenhain’s hematoxylin
  3. tungsten hematoxylin
  4. copper hematoxylin
36
Q

made up of ferric chloride and standard iron hematoxylin

A

weigert’s hematoxylin

37
Q

used in demonstrating muscle fibers and connective tissues

A

weigert’s hematoxylin

38
Q

combined with Van Gieson’s stain: collagen

A

weigert’s hematoxylin

39
Q

mordant of Weigert’s hematoxylin

A

ferric chloride

40
Q

how many days will weigert’s hematoxylin remain active

A

1-2 days

41
Q

It changes color from a deep blue-black violet, through violet, purple, brown, and yellowish brown

A

weigert’s hematoxylin

42
Q

popular cytologic stain, especially for the study of mitosis

A

Heidenhain’s hematoxylin

43
Q

recommended for mitochondria, muscle striation, chromatin, and myelin

A

Heidenhain’s hematoxylin

44
Q

mordant for heidenhain’s hematoxylin

A

Ferric Ammonium sulfate

45
Q

variant of Mallory’s PTAH

A

Tungsten hematoxylin

46
Q

recommended for staining muscle striation

A

Tungsten hematoxylin

47
Q

used for the study of spermatogenesis

A

Copper hematoxylin

48
Q

routinely used as counterstain after hematoxylin and before methylene blue

A

eosin

49
Q

red acidic dye in 3 forms

A
  • Eosin Y
  • Eosin B
  • Eosin S
50
Q

alcohol soluble eosin

A

Eosin S

51
Q

Yellowish and most commonly used eosin

A

Eosin Y

52
Q

Color of nuclei

A

Blue to blue black

53
Q

Color of decalcified bone matrix, collagen, osteoid

A

Pink

HSTCYT1 (18 decks)

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