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HSTCYT1 > Lab Fixation > Flashcards

Lab Fixation Flashcards

1
Q

Preparation, processing, and staining of tissue sections for microscopic study to be interpreted by the pathologist

A

Histotechniques

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2
Q

Study of disease at the tissue level

A

Histopathology

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3
Q

Removal of cells from the area of abnormality

A

Fine needle aspiration

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4
Q

Considered as the simplest and least invasive method of collecting biopsy specimens

A

Fine needle aspiration

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5
Q

Method of collection for fluid-containing tumors

A

Fine needle aspiration

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6
Q

Removal of cells and small amount of surrounding tissue

A

Core needle biopsy

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7
Q

Removal of cells and small amount of surrounding tissue

A

Core needle biopsy

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8
Q

Removal of cells with more surrounding tissue; for dermatological samples

A

Incisional biopsy

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9
Q

Removal of the entire area in question

A

Excisional biopsy

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10
Q

Removal of 3-4mm cylindrical core of tissue samples

A

Punch biopsy

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11
Q

Measurement of small punch biopsy

A

2mm

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12
Q

Measurement of large punch biopsy

A

4mm

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13
Q

Removal of small fragments of tissue from a surface

A

Shave biopsy

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14
Q

Removal of tissue or growths from body cavities

A

Curettings

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15
Q

Where shoul the lesion be for punch biopsy

A

Center of area in question

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16
Q

Scoon or spoon sample

A

Curettings

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17
Q

Superior than clinical lab test

A

Biopsy

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18
Q

Storage of specimen

A

1month to 1 year

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19
Q

Storage time of tissue blocks

A

3-10 years

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20
Q

Storage time for slides

A

Indefinite

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21
Q

Storag etime for records (request and result forms)

A

Permanent

22
Q

What types of dyes are used to stain fresh tissue examination

A

Supravital or differential

23
Q

What types of microscopes are used to examine fresh tissue examination

A

Brightfield or phase contrast microscope

24
Q

No fixative is required

A

Fresh tissue examination

25
Q

Advantages of fresh tissue examination

A

-observation of physiologic processes or protoplasmic activities (motion, mitosis, phagocytosis, pinocytosis)
-relatively simple and easy to perform

26
Q

Disadvantages of fresh tissue examination

A

-limited use
-liable to develop changes observed after death (putrefaction and autolysis)

27
Q

Dissection or separation of tissue components in NSS or Ringer’s solution

A

Teasing

28
Q

Examined as stained or unstained and anatomical relationship is destroyed

A

Teasing

29
Q

Tissue is sandwhiched between2 sides

A

Squash

30
Q

Stain is applied on one side of th slide and allowed to spread

A

Squash

31
Q

What type of spreading action is observed in squah preparation

A

Capillary action

32
Q

For cytological studies, especially for th diagnosis of cancer

A

Smearing

33
Q

For sections and sediments, performed using a wire loop, applicator stick or another slide

A

Smearing

34
Q

Uniform distribution in a zigzag manner

A

Streaking

35
Q

For thick or mucoid specimens which involves teasing on a slide

A

Spreading

36
Q

Maintains intercellular relationship

A

Spreading

37
Q

For the preparation of blood and bone marrow smears

A

Pull-apart

38
Q

For thick secretions resulting to an even distribution of sample

A

Pull-apart

39
Q

One side of a slide is allowed to touch a surface of the sample

A

Touch preparation

40
Q

Preparedusing freezing microtome or cryostat

A

Frozen section

41
Q

For rapid diagnosis

A

Frozen section

42
Q

Specimen accessioning or identification is performed by

A

Medical technologist

43
Q

Gross examination and sampling is performed by

A

Pathologist

44
Q

osmolality of light microscopy

A

slightly hypertonic (400-450 mOsm)

45
Q

osmolality of electron microscopy

A

more or less isotonic (340 mOsm)

46
Q

what does tap water remove

A

-chromates
-formalin
-osmic acid

47
Q

decolorizer of mercuric chloride

A

5% na thiosulfate

48
Q

decolorizer of melanin

A

potassium permanganate KMnO4

49
Q

what reduces melanin

A

pyrogallic acid

50
Q

bleach and final removal of melanin

A

H2O2

HSTCYT1 (18 decks)

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