EMBEDDING/CASTING/BLOCKING Flashcards
Arranging tissue in the molds or fixing the tissue block on the microtome
Orientation
Arranging the tissue ribbons on the slide
Orientation
2 L shaped heavy metal arranged flat on a metal surface
Leuckhart’s embedding mold
With several interlocking plates making several compartments
Compound E unit
Types of disposable molds
*peel-away
*plastic ice trays
*paper boats
base mold is fitted to base ring and the base ring serves as the block holder
Plastic embedding rings and base molds
uses tissue- tek system
Plastic embedding rings and base molds
At what temp should paraffin mtd be adjusted when melted and poured
Greater than 5-10C above MP
Embed tissue in shallow tin of enamel pans, cover with sheets of weighted glass
Celloidin/ nitocellulose mtd
Tissue is first infiltrated with celloidin then ebedded in paraffin
Double embedding
Its purpose is to cut large blocks for dense or firms tissues
Double embedding