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Infectious Agents > Lab Quiz: Lab 2 > Flashcards

Lab Quiz: Lab 2 Flashcards

1
Q

What is complex media?

A

Those that can not be chemically defined

They usually contain some type of biological fluid or component

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2
Q

What are examples of complex media?

A

Blood
Serum
Bile salts
Different enzymatic digests of protein

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3
Q

What is chemically defined media?

A

Those in which every component is chemically known and added at a defined concentration

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4
Q

What does differential media have added to it?

A

Some type of indicator or indicatory system

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5
Q

What are examples of differential media?

A

Blood for hemolysis detection

Specific carbohydrates and a pH indicating dye

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6
Q

What does selective media have added to it?

A

Some type of inhibitor to prevent the growth of certain organisms

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7
Q

What are examples of selective inhibitors?

A 
Antibiotics
High salt concentrations
Bile salts
Growth inhibiting dyes (crystal violet)
Limiting nutrients
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8
Q

What is blood agar?

A

A universal complex differential medium used for the isolation and cultivation of pathogenic, and especially fastidious, microorganisms

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9
Q

What does blood agar support the growth of and detect?

A

Hemolysis by most medically important bacteria, yeasts, and fungi

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10
Q

What is trypticase soy agar?

A

A base medium to which 5% defibrillated sheep blood is added once the base medium is cooled to 50ºC

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11
Q

How much of the medium is in a standard 150 mm trypticase soy agar plate?

A

20 mL

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12
Q

What is colistin-nalidixic acid (CNA) agar?

A

Blood agar to which the antibiotics colistin and nalidixic acid have been added to preclude the growth of gram-negative organisms

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13
Q

What is colistin?

A

A peptide antibiotic

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14
Q

What is nalidixic acid?

A

A quinolone acid

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15
Q

What does CNA generally replace?

A

Phenyl Ethyl Alcohol (PEA)

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16
Q

What will grow on CNA agar?

A

Gram-positive organisms

17
Q

What is MacConkey agar?

A

A moderately selective medium for the isolation and presumptive identification of enteric bacteria from feces and urine

18
Q

What is MacConkey agar useful for?

A

Isolation and separation of swarming Proteus from other enteric organisms
Differentiating certain non-enteric gram-negative organisms in lab diagnosis

19
Q

What do the peptones in MacConkey agar provide?

A

Nitrogenous nutrients and amino acids

20
Q

What do coliform organisms, which ferment lactose develop into with MacConkey agar?

A

Red/pink colonies surrounded by a zone of precipitated bile

21
Q

What do lactose nonfermenters develop into with MacConkey agar?

A

Colorless, transparent colonies

22
Q

What do bile alts and crystal violet do in MacConkey agar?

A

Inhibit gram-positive and many gram-negative bacteria

23
Q

What can grow on MacConkey agar?

A

Only certain types of gram-negative organisms

Infectious Agents (38 decks)

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