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Microbio > LAB MIDTERM TESTS > Flashcards

LAB MIDTERM TESTS Flashcards

1
Q

identifies bacterium that can ferment glucose

A

purpose of Carbohydrate (Glucose) Fermentation test:

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2
Q

broth turns YELLOW

A

positive carbohydrate test

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3
Q

broth stays RED

A

negative carbohyrate test

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4
Q

phenol red

A

what acts as a pH indicator in carbohydrate test?

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5
Q

lowers pH below 7 (or 6.8)

A

carbohydrate test: if a bacterium ferments a sugar, what will happen to the pH of the medium?

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6
Q

determines which fermentation route is used to utilize glucose; detects the production of acids (lowers pH)

A

purpose of Mixed Acid (MR) test:

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7
Q

RED broth at a pH of 5 or less

A

pos. MR test

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8
Q

broth stays YELLOW

A

neg. MR test

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9
Q

Methyl Red

A

reagent used in MR test

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10
Q

pH of 5 or lower

A

when does methyl red turn red in the MR test?

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11
Q

determines which fermentation route is used to utilize glucose; detects precursors of Butanediol

A

purpose of Voges-Proskauer (VP) test:

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12
Q

RED broth

A

pos. VP test

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13
Q

broth stays YELLOW

A

neg. VP test

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14
Q

Barritt’s A and Barritt’s B reagent

A

reagents used in VP test:

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15
Q

precursors of Butanediol

A

what does Barritt’s A and B reagent detect in VP test?

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16
Q

identifies bacterium that use citrate as a carbon source and form sodium carbonate (Na2CO3)

A

purpose of Citrate Agar test:

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17
Q

blue agar

A

pos. Citrate test

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18
Q

green agar

A

neg. Citrate agar test

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19
Q

bromothymol blue

A

what acts as pH indicator in Citrate test?

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20
Q

green

A

citrate test: pH indicator bromothymol blue in a NEUTRAL pH

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21
Q

blue

A

citrate test: color of bromothyl blue in an ALKALINE pH

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22
Q

identifies bacterium that possess the enzyme catalase (breaks down harmful hydrogen peroxide (HOOH) into water and oxygen

A

purpose of Catalase test:

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23
Q

break hydrogen peroxide into water and oxygen

A

what does catalase enzyme do?

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24
Q

bubbles

A

pos. Catalase test

25
Q

no bubbles

A

neg. Catalse test

26
Q

hydrogen peroxide

A

reagent used for Catalse test

27
Q

used to identify bacterium that produce cytochrome c oxidase (part of electron transport system); means they perform aerobic respiration to obtain energy

A

purpose of Oxidase test:

28
Q

PURPLE stain on streak

A

pos. Oxidase test

29
Q

no color change

A

neg. Oxidase test

30
Q

oxidase reagents

A

reagents used for Oxidase test

31
Q

aerobic respiration

A

what type of respiration does a bacterium use if it has cytochrome c oxidase?

32
Q

identifies bacteria that can hydrolyze starch; produce AMYLASE if they do

A

purpose of Start Hydrolysis test:

33
Q

clear zone around bacteria (means starch was DIGESTED); does NOT turn black from iodine

A

pos. starch hydrolysis test

34
Q

area around bacteria turns black (means starch was NOT digested)

A

neg. starch hydrolysis test

35
Q

Iodine

A

reagent used for Start Hydrolysis test:

36
Q

turns areas where starch was NOT digested black and leaves areas where starch WAS digested clear

A

how does iodine work in the Starch Hydrolysis test?

37
Q

identifies bacteria than can hydrolyze casein (makes milk white); uses skim milk agar plate

A

purpose of Casein Hydrolysis test:

38
Q

agar turns CLEAR

A

pos. Casein test

39
Q

no color change of agar

A

neg. Casein Hydrolysis test

40
Q

none

A

reagent used for Casein Hydrolysis test

41
Q

Sulfide Indole Motility

A

what does SIM stand for?

42
Q

identifies bacteria that produce hydrogen sulfide (through agar color)

A

purpose of H2S (hydrogen sulfide) Production:

43
Q

BLACK agar

A

pos. H2S (hydrogen sulfide) Production

44
Q

no color change

A

neg. H2S (hydrogen sulfide) Production

45
Q

none

A

reagent used for H2S production test:

46
Q

amylase

A

if bacteria can hydrolyze starch, what enzyme do they produce?

47
Q

identifies bacteria that produce indole (indole pyruvic acid); means they are capable of breaking down tryptophan

A

purpose of Tryptophan Degradation (part of SIM tube test):

48
Q

Kovac’s reagent turns RED

A

pos. Tryptophan Production test

49
Q

Kovac’s reagent remains yellow

A

neg. Tryptophan Production test

50
Q

detects the presence of Indole

A

what does Kovac’s reagent do in the Tryptophan Production test?

51
Q

tryptophan

A

what amino acid are bacteria capable of breaking down if they are positive for the Indole test?

52
Q

identifies bacteria that produce the enzyme Urease and perform Urea Hydrolysis; break down urea into CO2 + ammonia (NH3)

A

purpose of Urease test:

53
Q

HOT PINK broth (nikki minaj bby) when pH increase

A

pos. Urease test

54
Q

broth remains yellow

A

neg. Urease test

55
Q

phenol red

A

what is used as a pH indicator in Urease test?

56
Q

8.1

A

what pH does phenol red turn bright pink in the Urease test?

57
Q

CO2 + ammonia

A

produces of urea hydrolysis =

58
Q

INCREASE pH

A

if a bacterium produces urease, what happens to the pH of the culture medium?

Microbio (21 decks)

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