Lab Diagnosis Of Viral Infections

Question 1

Why we nede to diagnose viral infections

Answer 1

Result in increasing demand for rapid detection methods, viral load testing, antiviral susceptibility, genotyping

Question 2

When we need to diagnose viral infections

Answer 2

When treatment and prognosis depend entirely on correct diagnosis
When there is an epidemic spread of a viral disease
When viral disease can be treated with antiviral drugs

Question 3

Virus detection is related with

Answer 3

Appropriate collection site and method
Proper timing of specimen collection
Effective and timely processing of sufficient specimen

Question 4

Which one is more stable noneneveloped viruses or enveloped viruses

Answer 4

Noneneveloped viruses are more stable

Question 5

Viral disease diagnosis depends on 3 main principles

Answer 5

Direct detection
Isolation
Serology

Question 6

Direct detection of virus done by

Answer 6

Viral particle detection
Viral specific ag detection
Viral nucleic acid detection
Cytopathological changes

Question 7

What is the biggest cons of Electron microscopy

Answer 7

Low sensitivity

Question 8

For viral ag detection which tests are used

Answer 8

Immunofluorescence ab test
ELISA test — direct detection
Immunochromothrophy test

Question 9

Viral nucleic acid detection

Answer 9

PCR — direct detection of virus

Question 10

Types of cell cultures

Answer 10

Primary — from freshly killed
Semi-continuous cell culture— from embryonic tissues
Continuous cell culture— immortalized cells from tumors

Question 11

Detection of virus growth in cell cultures

Answer 11

Cytopayhic effects
Metabolic inhibition
Hemadsorption
Interference
Transformation

Question 12

Diagnostic criteria for serological tests

Answer 12

Rising titer
Single serum sample for IgM detection
Seroconversion IgMİ IgG
Single serum sample for high titer IgG —> not reliable *****