Cultivation And Identification Of Bacteria

Question 1

First screening step in identification

Answer 1

Organisms microscopic morphology and staining characteristics

Question 2

Wet mount preparation

Answer 2

Easy,Rapid
1-Collect sample from container
2-Add sample to slide
3-Place cover slip on sample

Question 3

Steps of preparing a bacterial smear

Answer 3

1- smear of bacteria —> drop of a broth, colony from agar medium
2- smear is dried in air
3- slide is heat-fixed

Question 4

Simple stain procedure

Answer 4

Useage of only 1 stain—> methylene blue

Question 5

Most common and staining procedure

Answer 5

Gram staining

Question 6

Steps of gram staining

Answer 6

1-Fixation
2- crystal violet
3-Iodine treatment
4-Decolorization
5- Counter stain fuchsin

Question 7

Decolorization done with which substance for how long

Answer 7

Alcohol
10 seconds

Question 8

Crystal violet and Iodine is used for how long

Answer 8

1 minute

Question 9

Basic fuchsin for how long

Answer 9

30 seconds

Question 10

By the help of gram-staining procedure we can observe

Answer 10

Gram properties of the bacteria
Morphology of the bacteria

Question 11

When some bacterias can not be satined by gram staining bc of their cell-wall which staining can be use

Answer 11

Acid fast satining

Question 12

Which satins used in AFS

Answer 12

Methylene blue
Carbon Fuchsin

Question 13

Ziehl Neelsen is type of which satining

Answer 13

Acid Fast —> methylene blue

Question 14

Nocardia Asteroides

Answer 14

A type of Acid fast staining

Question 15

TB Auromine/Rhodamine

Answer 15

Acid fast stain

Question 16

Lactophenol cotton blue

Answer 16

Used in wet mount procedure
Visualization of fungal structures

Question 17

Indian ink for

Answer 17

Cryptococcus neoformans—> fungi

Question 18

What is cultivation

Answer 18

Process of growing microorganisms by taking them from infection site and grow them on artificial environment

Question 19

Common ingredients of media

Answer 19

Agar
Peptone
Yeast extract
Meat extract
Water
NaCl

Question 20

2 types of media

Answer 20

Enriched
Selective

Question 21

Types of enriched media

Answer 21

Sheep blood agar
Chocolate agar

Question 22

Types of selective media

Answer 22

MacConkey
Hektoen Enteric agar
Thayer Martin

Question 23

2 ways for Inoculation on Agar media

Answer 23

1- Striking method
2- Quantitative

Question 24

Chocolate agar contains

Answer 24

X (hemin)
V( NAD) factors

Question 25

Mac Conkey agar contains

Answer 25

Lactose
Bile salts

Question 26

Identification of gram positives done by

Answer 26

Catalase
Coagulase