Lab 9- Brucella

Question 1

Habitat

Answer 1

Infected host

Question 2

Morphology

Answer 2

0.6-1,5 um coccoid rods

Question 3

Staining

Answer 3

Gram -

Köster staining 
-3% safranin 3-5 min 
0,5% h2so4 
Washing
Methylene blue 2 min 

Modified Ziehl-neelsen staining

Question 4

Culture

Answer 4

Obligate aerobic 
Fastidious (vit.+ yeast extract)
Co2
R+s colonies 
S= b. Melitensis, b. Abortus, b. Suis 
R= b. Canis, b. Ovis 

Selective culture:
Polymyxin, bacitracin, cycloheximide

Question 5

Biochem

Answer 5

Catalase +
Oxidase +
Urease +

Question 6

Antigens

Answer 6

Close relationship

Cross reaction within the s and r colonies types

Cell wall: A and M polysaccharides

Question 7

Groups

Answer 7

Phage susceptibility
Oxidative metabolism
Natural host

Question 8

Biotype

Answer 8

Co2 demand
H2S
Fuchsin/ thionin tolerance
Agglutination

Question 9

Resistance

Answer 9

Medium

Question 10

Pathogenicity

Answer 10

Facultative intracellular bacterium

Question 11

Species

Answer 11

B. Melitensis : goat, sheep
B. Abortus: cattle
B. Suis : swine, heard, reindeer, rodents 
B. Pinnipediales : skal, walrus 
B. Ceti : whale, dolphins 
B. Ovis : sheep
B. Canis : dog
B. Neotomae : desert rat
B. Microti : vole