Lab-4 Erysipelothrix

Question 1

Habitat

Answer 1

Alimentary tract (swine)
Mud
Slime of fish
Water

Question 2

Morphology

Answer 2

1-3 um
rods (S-type)
Chains, filaments (R-type)

Question 3

Staining

Answer 3

Gram positive

Question 4

Culture

Answer 4

Nutrient agar
Replication 5-42 degrees
pH 6.7-9.2

Blood agarm alpha hemolysis in some species
Broth:
S-type; uniform turbidity
R-type; sediment

Selective culture
NaN3 + crystal violet
Neomycin

Question 5

Biochem

Answer 5

Catalase -
Oxidase -
Fermentative

Extracellular enzymes:
Neuraminidase: binding to endothelial cells
Hyaluronidase: spreading

Question 6

Antigens

Answer 6

Two common protein antigens for protection

Type specific polysaccharide haptens

Question 7

Resistance

Answer 7

Good, halotolerance (adaption to living in high salinity environment, ex. salty lakes), soil

Question 8

Pathogenicity

Answer 8

Facultative pathogenic

Swine: erysipelas
Sheep: wound infection
Birds: septicaemia
Humans: wound infection (erysepeloid)

Question 9

E. Tonsillarum

Answer 9

No biological difference
Serotype 7, and 6 others
Tonsils of swine
Endocarditis in dog

Question 10

Species of erysipelothrix?

Answer 10

Erysipelothrix rhusiopathiae

E. Tonsillarum

Question 11

What disease does E. Erysipelothrix cause in pigs?

Answer 11

Diamond skin

Question 12

What is important in swine, bird and mammals?

Answer 12

Can be in feces, soil might be contaminated

Question 13

Fish

Answer 13

Zoonotic agents-> humans (wide spectrum)

Question 14

Shape

Answer 14

Rod shape, thin + curved rods

Question 15

What is special about s-type colonies?

Answer 15

Specific polysaccharides

Question 16

Morphology 2

Answer 16

No capsule
No flagella
Non motile
No spore

Question 17

What kids special about r-shaped colonies?

Answer 17

Sometimes chain formation (chronic pathological shape)

Question 18

Hemolysis

Answer 18

Incomplete alpha hemolysis

Question 19

Medium supplements

Answer 19

Neomycin= ab that inhibits growth of unwanted bacteria

Question 20

What can it produce from S containing amino acids?

Answer 20

H2S

Question 21

Resistancy

Answer 21

Can tolerate high osmotic pressure 8,5 % NaCl

Can survive 1 month in soils

Question 22

Where can it be found in “resting state”.

Answer 22

Mucous membranes causing no pathogenic damage. Can be activated by stress or animal transport