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Bacteriology > Lab 4- Listeria > Flashcards

Lab 4- Listeria Flashcards

1
Q

Habitat

A

Soil, sewage, plants, silage, gut and food

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2
Q

Morphology

A

1-2 um thick rods

Flagella - 22 degrees

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3
Q

Staining

A

Gram positive

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4
Q

Culture

A

Simple
B-haemolysis
4-45 degrees
pH: 5.5-9.6

Selective: cattle serum, trypaflavin, nalidixic acid

Selective enrichment:
Cold enrichment; 4 degrees
U-tube

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5
Q

Biochemistry

A

Catalase +
Oxidase -
Fermentative

Virulence factors:
Listeriolysin O ( haemolysin): intracellular replication !
Monocytosis factor: cell wall, lipoid

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6
Q

Antigens

A

Cell wall polysaccharides
Flagellar protein
13 serotypes, most common: 1/2a, 4b

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7
Q

Resistance

A

Months in soil

Propagation in soil !

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8
Q

Pathogenicity

A

Facultative pathogenic

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9
Q

Virulence

A

Haemolysis
Lipolysis
Monocytosis factor
Cell wall lipoids

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10
Q

Pathogenicity in different species.

A 
Sheep: mainly CNS signs 
Cattle: mainly abortion 
Rabbit: septicaemia, abortion 
Humans: abortion, newborn septicaemia, encephalitis 
Birds: septicaemia
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11
Q

Species

A

L. Monocytogens
L. Ivanovoii

Factulative intracellular agents
Saprophytic species

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12
Q

L. Ivanovii

A

Better hemolysis

Serotype 5

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13
Q

When can the bacteria be found flagellated?

A

At room temp !

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14
Q

What is special about the motility ?

A

Tumbling motility (rolls)

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15
Q

What kind of hemolysis does it cause?

A

Weak, complete beta hemolysis

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16
Q

Can listeria propagate in the fridge ?

A

Yes, 4-45 degrees

17
Q

What is important concerning listeria and silage production?

A

The ph of the silage must be under 5.5, or the bacteria will spread in the fermented feedstuff

18
Q

How can you differentiate (alternative method) listeria from other bacteria’s?

A

Leave in fridge for several weeks, only listeria will survive

19
Q

Causes what disease ?

A

Listeriosis

20
Q

Symptoms of CNS damage ivy listeria in sheep?

A

Tilting of head, paresis of tongue, salivation (also ru)

21
Q

L. Monocytogens cause what disease in ov and ru ?

A

Encephalitis (circling disease), abortion, septicaemia

22
Q

L. Monocytogens cause what disease in ru?

A

Mastitis

23
Q

L. Monocytogens cause what disease in hu?

A

Septicaemia, abortion

Mainly from raw milk, vegetables, plants

24
Q

L. Monocytogens cause what disease in fe, ca, eq?

A

Abortion, encephalitis

Bacteriology (81 decks)

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