Lab 10- Mycoplasma Genus

Question 1

Habitat

Answer 1

Human, mucous membranes of animals (resp. Tract, genitals, intestine), plants

Question 2

Morphology

Answer 2

Smallest free living microorganisms 0.2-0.8 um
Pleomorphic (can change their shape), pear shaped, long branching filaments

(Chlamydia not free living, but also very small !)

Question 3

Culture

Answer 3

Fastidious
Horse serum (sterols, FA)
Yeast extract, DNA, lactalbumin, hydrolysate, urea, cysteine

No turbidity!

Time to form colony: 7-14d, needs CO2

Selective isolation: penicillin, tallium acetate

Some species cannot be cultured:
M. Suis, m. Ovis, m. Haemocanis, m. Haemofelis

Question 4

Biochem

Answer 4

Glucose fermentation, arginine hydrolysis

Cholesterol demand

Question 5

Antigens

Answer 5

Growth inhibition test

Metabolism inhibition test

Question 6

Classification

Answer 6

Family: genome size, cholesterol demand, morphology

Genus: optimal temp., ph, glucose, arginine, urease

Question 7

Pathogenicity

Answer 7

Saprophytes, faculative pathogens, pathogens

Question 8

Important mycoplasma species

Answer 8

Human: m. Pneumonia= pneumonia

Cattle:
m. Mycosides ssp. Mycosides = contagious bovine pleuropneumoniae

M. Bovigenitalium = pneumonia, genitals

M. Bovis = arthritis, mastitis, pneumonia

M. Dispar = pneumonia in calves

M. Bovoculi = conjunctivitis

M. Alifornicum (?) + m. Canadense = mastitis

Question 9

Species targeting sheep and goat

Answer 9

M. Capricolum ssp capripneumoniae = goat contagious pleuropneumoniae

M. Capricolum ssp capricolum = sheep, goat pneumonia, mastitis, arthritis, septicaemia

M. Mycoides ssp capri = goat

M. Agalactiae = sheep, goat pneumonia, contagious agalactia, arthritis

M. Conjunctivae = sheep, goat, conjunctivitis, keratoconjunctivitis

M. Ovipneumoniae = sheep pneumonia, keratoconjunctivitis

Question 10

Species targeting swine

Answer 10

M. Hyopneumoniae = enzootic pneumonia

M. Hyorhinis = polyserositis, rhinitis, arthritis

M. Hyosynoviae = arthritis

M. Suis = anaemia

Question 11

Species targeting dogs and cats

Answer 11

M. Haemocanis= dog anaemia, haemobartonellosis

M. Haemofelis = cat anaemia, haemobartonellosis

M. Felis conjunctivitis cat

M. Cyanos = respiration infection dog

Question 12

Species targeting poultry

Answer 12

M. Gallisepticum = chronic respiratory disease

M. Synoviae = synovitis (poultry + turkey)

M. Meleagridis, m. Anatis, m. Aseris = air sacculitis