Lab 4- Corynebacterium Flashcards
Habitat
Mucous membranes, skin, soil, milk (animal pathogens-saprophytes)
Morphology
Polymorphism, pleomorphism (coryneform)
Single, palisade, Chinese letters
Metachromatic granules (stains with different color)
Staining
Gram positive
Slightly acid-fast
Culture
Blood agar
Weak growth on nutrient agar
Hemolysis (sometimes delayed)
Different colony morphology
Biochem
Catalase +
Oxidase -
Fermentative
Toxin;
C. Diphtheriae
C. Pseudotubercolosis
Antigens
Complex
Resistance
Average
Pathogenicity
Local suppuration
Arthritis
Metritis
Mastitis
Species
C. Diphtheriae C. Pseudotuberculosis C. Renale C. Pilosum C. Cystitidis C. Bovis C. Kutscheri
C. Diphtheriae
Strains infected with temperate phage:
Toxin production
Diphthery
Vaccination
Strains not infected with temperate phage:
Pharyngitis, endocarditis
C. Pseudotubercolosis
Polymorphism, fimbria
Haemolysis
Virulence factors:
Exotoxin: phospholipase-D
Lipoids in the cell wall
Nitrate negative biotype:
Sheep, goat: pseudotubercolosis (caseous lymphodenitis), abortion, arthritis
Nitrate positive biotype:
Horse: equine ulcerative lymphangitis
What disease does c. Renale cause?
Cattle, horse, swine: pyelonephritis, cystitis
What disease does c. Pilosum cause?
Cattle: cystitis, pyleonephritis
What disease does c. Cystitidis cause?
Cattle: haemorrhagic cystitis
What disease does c. Bovis cause?
mastitis, abortion
What is the target species of c. Kutscheri?
Rodents
Motility
Non-motile
Hemolysis?
Weak beta hemolysis
What is the main target species for c. Pseudotubercolosis?
Eq, ov, goat
C. Diphtheriae is a ?
Human pathogen
What does temperate phage mean?
Encoded exotoxins
Characteristics of Nitrate negative species
Cannot reduce nitrate
What does CLA stand for and what is important about it?
Caseous (cheesy) lymphadenitis
Can survive for months in the environment
C. Renale, c. Pilosum, c. Cystitidis cause?
Urinary tract infections
C. Bovis is a ?
Mastitis pathogen
