Lab 4- Corynebacterium

Question 1

Habitat

Answer 1

Mucous membranes, skin, soil, milk (animal pathogens-saprophytes)

Question 2

Morphology

Answer 2

Polymorphism, pleomorphism (coryneform)
Single, palisade, Chinese letters
Metachromatic granules (stains with different color)

Question 3

Staining

Answer 3

Gram positive

Slightly acid-fast

Question 4

Culture

Answer 4

Blood agar
Weak growth on nutrient agar
Hemolysis (sometimes delayed)
Different colony morphology

Question 5

Biochem

Answer 5

Catalase +
Oxidase -
Fermentative

Toxin;
C. Diphtheriae
C. Pseudotubercolosis

Question 6

Antigens

Answer 6

Complex

Question 7

Resistance

Answer 7

Average

Question 8

Pathogenicity

Answer 8

Local suppuration
Arthritis
Metritis
Mastitis

Question 9

Species

Answer 9

C. Diphtheriae 
C. Pseudotuberculosis
C. Renale
C. Pilosum 
C. Cystitidis
C. Bovis 
C. Kutscheri

Question 10

C. Diphtheriae

Answer 10

Strains infected with temperate phage:
Toxin production
Diphthery
Vaccination

Strains not infected with temperate phage:
Pharyngitis, endocarditis

Question 11

C. Pseudotubercolosis

Answer 11

Polymorphism, fimbria
Haemolysis

Virulence factors:
Exotoxin: phospholipase-D
Lipoids in the cell wall

Nitrate negative biotype:
Sheep, goat: pseudotubercolosis (caseous lymphodenitis), abortion, arthritis

Nitrate positive biotype:
Horse: equine ulcerative lymphangitis

Question 12

What disease does c. Renale cause?

Answer 12

Cattle, horse, swine: pyelonephritis, cystitis

Question 13

What disease does c. Pilosum cause?

Answer 13

Cattle: cystitis, pyleonephritis

Question 14

What disease does c. Cystitidis cause?

Answer 14

Cattle: haemorrhagic cystitis

Question 15

What disease does c. Bovis cause?

Answer 15

mastitis, abortion

Question 16

What is the target species of c. Kutscheri?

Answer 16

Rodents

Question 17

Motility

Answer 17

Non-motile

Question 18

Hemolysis?

Answer 18

Weak beta hemolysis

Question 19

What is the main target species for c. Pseudotubercolosis?

Answer 19

Eq, ov, goat

Question 20

C. Diphtheriae is a ?

Answer 20

Human pathogen

Question 21

What does temperate phage mean?

Answer 21

Encoded exotoxins

Question 22

Characteristics of Nitrate negative species

Answer 22

Cannot reduce nitrate

Question 23

What does CLA stand for and what is important about it?

Answer 23

Caseous (cheesy) lymphadenitis

Can survive for months in the environment

Question 24

C. Renale, c. Pilosum, c. Cystitidis cause?

Answer 24

Urinary tract infections

Question 25

C. Bovis is a ?

Answer 25

Mastitis pathogen