Lab 3- Streptococcus

Question 1

Habitat

Answer 1

Skin, mucous membranes, food, milk, digestive tract

Question 2

Morphology

Answer 2

Coccus, chain

Capsule: hyaluridic acid, polysaccharide (some species)

Question 3

Staining

Answer 3

Gram positive

Question 4

Culture

Answer 4

Fastidious: blood agar, serum agar
Selective: crystal violet, thallium-SO4, esculin, blood

Question 5

Hemolysis

Answer 5

Alpha hemolysis
Beta hemolysis
No hemolysis

Question 6

Biochem

Answer 6

Catalase -
Oxidase -
Fermentative

Utilization of glycosides:
Esculin, salicin

Extracellular enzymes:
Fibrinolysin 
Hyaluronidase 
Nucleases 
Proteases 

Toxins

Question 7

Antigens

Answer 7

Complex

Lancefield A-W
Other type specific antigens 
Capsule antigens (s. Suis, s.pneumoniae)

Question 8

Resistance

Answer 8

Good

Question 9

Pathogenicity

Answer 9

Local suppuration
Metritis, mastitis, arthritis 
Septicaemia 
Strangles 
Pneumonia diseases

Question 10

Grouping

Answer 10

Hemolysis
Biochem characters
Antigens

Question 11

Species

Answer 11

S. Pyogenes
S. Agalactiae
S. Dysgalactiae
S. Uberis 
S. Dysgalactiae subspecies equisimilis
S. Equi subspecies equi 
S. Equi subspecies zooepidemicus 
S. Suis
S. Porcinus
S. Canis
S. Bovis
S. Salvarius 
S. Sanguis
S. Mutant
S. Pneumoniae

Question 12

Enterococcus culture

Answer 12

10-45 degrees
Ph 9,6
Lancefield D

Question 13

Enterococcus species

Answer 13

E. Faecalis
E. Faecium
E. Avium
E. Gallinarum

Question 14

Enterococcus habitat

Answer 14

Gut, mainly saprophytes

Question 15

Enterococcus causes?

Answer 15

Sometimes endocarditis and abscesses

Question 16

Lactococcus

Answer 16

Lancefield N
Fast lactose fermenters (homofermenters)
Gut flora (probiotic)
Dairy products (starter cultures: Bulgarian milk, butter)
Species:
L. Lactis subspecies lactis 
L. Lactis subspecies cremoris 
L. Plantarum: silage

Question 17

Anaerobic cocci

Habitat?

Answer 17

Mucous membranes

Saprophytes, sporadically inflammation

Question 18

Anaerobic cocci species ?

Answer 18

Peptococcus: metritis, arthritis, pneumonia
Peptostreptococcus : metritis, arthritis, pneumonia
Peptoniphilus: arthritis, septicaemia
Ruminococcus: saprophytes
Sarcina: saprophytes

Question 19

Lactobacillus

Habitat?

Answer 19

Mucous membranes, gut, food, feed. Plant

Question 20

Lactobacillus morphology ?

Answer 20

Long, thin, sometimes curved rods

Question 21

Lactobacillus staining ?

Answer 21

Gram positive

Question 22

Lactobacillus culture ?

Answer 22

Microaerophilic
Optimal temp 30-40 degrees (2-53 degrees)
Ph: 5.5-6.2

Question 23

Lactobacillus biochem

Answer 23

Active fermentation
Lactate ph 4.0
Silage

Question 24

Lactobacillus pathogenicity?

Answer 24

Saprophytes 
Mucous membranes (probiotic)
Dairy industry

Question 25

S. Pyogenes cause ?

Answer 25

Has capsule
Toxin
Human: scarlet fever, rheumatic fever, glomerulonephritis

Question 26

S. Agalactiae, s. Dysgalactiae and s. Uberis cause?

Answer 26

Affect cow and ruminants

Mastitis

Question 27

S. Dysgalactiae subspecies equisimilis cause?

Answer 27

Lymphadenitis and abortion (horse)

Question 28

S. Equi subspecies equi cause?

Answer 28

Horse strangles

Question 29

S. Equi subspecies zooepidemicus cause?

Answer 29

Suppuration, septicaemia, mastitis and abortion

Question 30

S. Suis cause?

Answer 30

Septicaemia, arthritis and meningitis

Question 31

S. Porcinus cause?

Answer 31

Lymphadenitis, abscesses

Question 32

S. Canis cause?

Answer 32

Metritis, neonatal septicaemia

Question 33

S. Bovis cause?

Answer 33

Septicaemia (pigeon)

Question 34

S. Salivarius, s. Sanguis, s. Mutans cause?

Answer 34

Oral streptococci

Question 35

S. Pneumoniae cause?

Answer 35

Affect calf, foal, human

Pneumonia, meningitis